Relationship between HLA Gene Polymorphism and Aplastic Anemia in Northern Chinese Han Patients.
- Author:
Bo-Jing WANG
1
;
Ya-Mei WU
1
;
Xiao-Hong LI
1
;
Li-Xin XU
1
;
Jing WANG
1
;
Bei YAN
1
;
Hai-Tao WANG
1
;
Song-Wei LI
1
;
Ya-Hui GAO
1
;
Tian-Tian ZHANG
1
;
Li WANG
1
;
Ya-Qian ZHANG
1
;
Xiao-Xiong WU
2
Author Information
- Publication Type:Journal Article
- MeSH: Alleles; Anemia, Aplastic; genetics; China; Gene Frequency; Genetic Predisposition to Disease; HLA Antigens; genetics; Humans; Polymorphism, Genetic
- From: Journal of Experimental Hematology 2018;26(6):1731-1737
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the relationship between HLA-A, -B, -C, -DRB1, -DQB1 gene polymorphism and aplastic anemia （AA）of 65 cases in Northern China.
METHODS:The high resolution genotyping of HLA-A, -B, -C, -DRB1, -DQB1 alleles in 65 AA patients and 772 healthy controls was performed with polymerase chain reaction-sequence specific oligonucleotide (PCR-SSO), the relationship between HLA-A, -B, -C, -DRB1, -DQB1 gene polymorphism and aplastic anemia was analyzed by Pearson Chi-square，Continuity Correction， Two-sided Fisher's Exact Test and Odds Ratio.
RESULTS:The HLA-B*1302（10% vs 4.21%）, B*3501（7.69% vs 3.89%）, DRB1* 0701（10% vs 4.73%）, DRB1*0901（19.23% vs 7.58%）, DQB1*0202（9.23% vs 3.76%） gene frequency in AA patients was higher than those in health controls, the difference was statistically significant (P<0.05), the χ were 9.049, 4.336, 6.838, 20.974 and 8.968, OR ratio was 2.528, 2.061, 2.239, 2.904 and 2.605. However, the HLA-A*3303（1.54% vs 6.93%）, DQB1*0302（1.54% vs 6.02%） gene frequency in AA patients was lower than those in healthy controls, the difference was statistically significant (P<0.05), the χ was 5.726 and 4.505, the OR ratio were 0.210 and 0.244.
CONCLUSION:The polymorphism of HLA-A, -B, -DRB1, -DQB1 alleles is associate with AA in these patient cases, the HLA-B*1302, HLA-B*3501, HLA-DRB1*0701, HLA-DRB1*0901 and HLA-DQB1*0202 may be sensitive genes to AA, while the HLA-A*3303 and HLA-DQB1*0302 may be protective genes on AA.
