Inulin Can Alleviate Metabolism Disorders in ob/ob Mice by Partially Restoring Leptin-related Pathways Mediated by Gut Microbiota.
10.1016/j.gpb.2019.03.001
- Author:
Xiaofeng SONG
1
,
2
,
3
;
Liang ZHONG
4
;
Na LYU
1
,
3
;
Fei LIU
1
,
3
;
Boxing LI
4
;
Yanan HAO
4
;
Yong XUE
5
;
Jing LI
1
,
3
;
Yuqing FENG
1
,
6
;
Yue MA
1
,
6
;
Yongfei HU
7
;
Baoli ZHU
1
,
2
,
8
,
9
Author Information
1. CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
2. Savaid Medical School, University of Chinese Academy of Sciences, Beijing 100049, China
3. Beijing Key Laboratory of Microbial Drug Resistance and Resistome, Beijing 100101, China.
4. CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
5. College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.
6. Savaid Medical School, University of Chinese Academy of Sciences, Beijing 100049, China.
7. State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China. Electronic address: huyongfei@cau.edu.cn.
8. Beijing Key Laboratory of Microbial Drug Resistance and Resistome, Beijing 100101, China
9. Department of Pathogenic Biology, School of Basic Medical Sciences, Southwest Medical University, Luzhou 646000, China. Electronic address: zhubaoli@im.ac.cn.
- Publication Type:Journal Article
- Keywords:
Gut microbiota;
Metabolic disorders;
Obesity;
Prebiotics;
Transcriptome
- MeSH:
AMP-Activated Protein Kinases;
metabolism;
Animals;
Cecum;
enzymology;
metabolism;
microbiology;
Gastrointestinal Microbiome;
drug effects;
Inulin;
therapeutic use;
Leptin;
genetics;
Male;
Metabolic Diseases;
drug therapy;
enzymology;
metabolism;
microbiology;
Mice;
Mice, Obese;
Prebiotics;
Signal Transduction;
drug effects;
Transcriptome
- From:
Genomics, Proteomics & Bioinformatics
2019;17(1):64-75
- CountryChina
- Language:English
-
Abstract:
Inulin has been used as a prebiotic to alleviate glucose and lipid metabolism disorders in mice and humans by modulating the gut microbiota. However, the mechanism underlying the alleviation of metabolic disorders by inulin through interactions between the gut microbiota and host cells is unclear. We use ob/ob mice as a model to study the effect of inulin on the cecal microbiota by 16S rRNA gene amplicon sequencing and its interaction with host cells by transcriptomics. The inulin-supplemented diet improved glucose and lipid metabolism disorder parameters in ob/ob mice, alleviating fat accumulation and glucose intolerance. The α diversity of gut microbial community of ob/ob mice was reduced after inulin treatment, while the β diversity tended to return to the level of wild type mice. Interestingly, Prevotellaceae UCG 001 (family Prevotellaceae) was obviously enriched after inulin treatment. A comparative analysis of the gene expression profile showed that the cecal transcriptome was changed in leptin gene deficiency mice, whereas the inulin-supplemented diet partially reversed the changes in leptin gene-related signaling pathways, especially AMPK signaling pathway, where the levels of gene expression became comparable to those in wild type mice. Further analysis indicated that Prevotellaceae UCG 001 was positively correlated with the AMPK signaling pathway, which was negatively correlated with markers of glycolipid metabolism disorders. Our results suggest that the inulin-supplemented diet alleviates glucose and lipid metabolism disorders by partially restoring leptin related pathways mediated by gut microbiota.
