H3K27me3 Signal in the Cis Regulatory Elements Reveals the Differentiation Potential of Progenitors During Drosophila Neuroglial Development.
10.1016/j.gpb.2018.12.009
- Author:
Xiaolong CHEN
1
;
Youqiong YE
1
;
Liang GU
1
;
Jin SUN
1
;
Yanhua DU
1
;
Wen-Ju LIU
1
;
Wei LI
2
;
Xiaobai ZHANG
1
;
Cizhong JIANG
3
,
4
Author Information
1. Institute of Translational Research, Tongji Hospital, School of Life Sciences and Technology, Shanghai Key Laboratory of Signaling and Disease Research, Tongji University, Shanghai 200092, China.
2. Tongji University Library, Tongji University, Shanghai 200092, China.
3. Institute of Translational Research, Tongji Hospital, School of Life Sciences and Technology, Shanghai Key Laboratory of Signaling and Disease Research, Tongji University, Shanghai 200092, China
4. Research Center of Stem Cells and Ageing, Tsingtao Advanced Research Institute, Tongji University, Tsingtao 266071, China. Electronic address: czjiang@tongji.edu.cn.
- Publication Type:Letter
- Keywords:
Glia;
Histone modification;
Neural stem cell;
Neuron;
Nucleosome
- From:
Genomics, Proteomics & Bioinformatics
2019;17(3):297-304
- CountryChina
- Language:English
-
Abstract:
Drosophila neural development undergoes extensive chromatin remodeling and precise epigenetic regulation. However, the roles of chromatin remodeling in establishment and maintenance of cell identity during cell fate transition remain enigmatic. Here, we compared the changes in gene expression, as well as the dynamics of nucleosome positioning and key histone modifications between the four major neural cell types during Drosophila neural development. We find that the neural progenitors can be separated from the terminally differentiated cells based on their gene expression profiles, whereas nucleosome distribution in the flanking regions of transcription start sites fails to identify the relationships between the progenitors and the differentiated cells. H3K27me3 signal in promoters and enhancers can not only distinguish the progenitors from the differentiated cells but also identify the differentiation path of the neural stem cells (NSCs) to the intermediate progenitor cells to the glial cells. In contrast, H3K9ac signal fails to identify the differentiation path, although it activates distinct sets of genes with neuron-specific and glia-related functions during the differentiation of the NSCs into neurons and glia, respectively. Together, our study provides novel insights into the crucial roles of chromatin remodeling in determining cell type during Drosophila neural development.
