Stability of a type 2 diabetes rat model induced by high-fat diet feeding with low-dose streptozotocin injection.

Xiao-xuan Guo; Yong Wang; Kai Wang; Bao-ping JI; Feng Zhou

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Stability of a type 2 diabetes rat model induced by high-fat diet feeding with low-dose streptozotocin injection.

Author: Xiao-Xuan GUO ¹ ; Yong WANG ² ; Kai WANG ³ ; Bao-Ping JI ¹ ; Feng ZHOU ¹
Author Information

1. Beijing Key Laboratory of Functional Food from Plant Resources, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.
2. Academy of State Administration of Grain, Beijing 100037, China.
3. Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, China.
Publication Type:Journal Article
Keywords: High-fat diet; Stability; Streptozotocin; Type 2 diabetes mellitus
MeSH: Animals; Blood Glucose; metabolism; Diabetes Mellitus, Experimental; blood; etiology; physiopathology; Diabetes Mellitus, Type 2; blood; etiology; physiopathology; Diet, High-Fat; adverse effects; Insulin; blood; Lipids; blood; Liver; drug effects; pathology; physiopathology; Male; Malondialdehyde; blood; Oxidative Stress; Rats; Rats, Wistar; Streptozocin; administration & dosage; toxicity; Superoxide Dismutase; blood; Uric Acid; blood
From: Journal of Zhejiang University. Science. B 2018;19(7):559-569
CountryChina
Language:English
Abstract: OBJECTIVE:The present study aims at determining the stability of a popular type 2 diabetes rat model induced by a high-fat diet combined with a low-dose streptozotocin injection.
METHODS:Wistar rats were fed with a high-fat diet for 8 weeks followed by a one-time injection of 25 or 35 mg/kg streptozotocin to induce type 2 diabetes. Then the diabetic rats were fed with regular diet/high-fat diet for 4 weeks. Changes in biochemical parameters were monitored during the 4 weeks.
RESULTS:All the rats developed more severe dyslipidemia and hepatic dysfunction after streptozotocin injection. The features of 35 mg/kg streptozotocin rats more resembled type 1 diabetes with decreased body weight and blood insulin. Rats with 25 mg/kg streptozotocin followed by normal diet feeding showed normalized blood glucose level and pancreatic structure, indicating that normal diet might help recovery from certain symptoms of type 2 diabetes. In comparison, diabetic rats fed with high-fat diet presented decreased but relatively stable blood glucose level, and this was significantly higher than that of the control group (P<0.05).
CONCLUSIONS:This model easily recovers with normal diet feeding. A high-fat diet is suggested as the background diet in future pharmacological studies using this model.