Neuroprotective effects of Ginkgo biloba extract and Ginkgolide B against oxygen-glucose deprivation/reoxygenation and glucose injury in a new in vitro multicellular network model.
10.1007/s11684-017-0547-2
- Author:
Xiaohan YANG
1
;
Tiezheng ZHENG
1
;
Hao HONG
2
;
Nan CAI
2
;
Xiaofeng ZHOU
2
;
Changkai SUN
3
;
Liying WU
4
;
Shuhong LIU
4
;
Yongqi ZHAO
4
;
Lingling ZHU
4
;
Ming FAN
5
;
Xuezhong ZHOU
6
;
Fengxie JIN
7
Author Information
1. Department of Biomedical Engineering, Faculty of Electronic Information and Electrical Engineering, Dalian University of Technology, Dalian, 116024, China.
2. Liaoning Provincial Key Laboratory of Cerebral Diseases, Institute for Brain Disorders, Dalian Medical University, Dalian, 116044, China.
3. Department of Biomedical Engineering, Faculty of Electronic Information and Electrical Engineering, Dalian University of Technology, Dalian, 116024, China. acksun110@vip.sina.com.
4. Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing, 100850, China.
5. Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing, 100850, China. bfanmingchina@126.com.
6. School of Computer and Information Technology and Beijing Key Lab of Traffic Data Analysis and Mining, Beijing Jiaotong University, Beijing, 100044, China. cxzzhou@bjtu.edu.cn.
7. College of Biotechnology, Dalian Polytechnic University, Dalian, 116034, China.
- Publication Type:Journal Article
- Keywords:
Ginkgo biloba extract;
Ginkgolide B;
acute ischemic stroke;
network model;
neuroprotection
- MeSH:
Animals;
Apoptosis;
drug effects;
Brain Ischemia;
drug therapy;
Cell Survival;
Cells, Cultured;
Disease Models, Animal;
Endothelial Cells;
drug effects;
Ginkgolides;
pharmacology;
Glucose;
Lactones;
pharmacology;
Neurons;
drug effects;
Neuroprotective Agents;
pharmacology;
Oxygen;
Plant Extracts;
pharmacology;
Rats;
Stroke;
drug therapy
- From:
Frontiers of Medicine
2018;12(3):307-318
- CountryChina
- Language:English
-
Abstract:
Acute ischemic stroke (AIS), as the third leading cause of death worldwide, is characterized by its high incidence, mortality rate, high incurred disability rate, and frequent reoccurrence. The neuroprotective effects of Ginkgo biloba extract (GBE) against several cerebral diseases have been reported in previous studies, but the underlying mechanisms of action are still unclear. Using a novel in vitro rat cortical capillary endothelial cell-astrocyte-neuron network model, we investigated the neuroprotective effects of GBE and one of its important constituents, Ginkgolide B (GB), against oxygen-glucose deprivation/reoxygenation and glucose (OGD/R) injury. In this model, rat cortical capillary endothelial cells, astrocytes, and neurons were cocultured so that they could be synchronously observed in the same system. Pretreatment with GBE or GB increased the neuron cell viability, ameliorated cell injury, and inhibited the cell apoptotic rate through Bax and Bcl-2 expression regulation after OGD/R injury. Furthermore, GBE or GB pretreatment enhanced the transendothelial electrical resistance of capillary endothelial monolayers, reduced the endothelial permeability coefficients for sodium fluorescein (Na-F), and increased the expression levels of tight junction proteins, namely, ZO-1 and occludin, in endothelial cells. Results demonstrated the preventive effects of GBE on neuronal cell death and enhancement of the function of brain capillary endothelial monolayers after OGD/R injury in vitro; thus, GBE could be used as an effective neuroprotective agent for AIS/reperfusion, with GB as one of its significant constituents.
