Oxidative stress in granulosa cells contributes to poor oocyte quality and IVF-ET outcomes in women with polycystic ovary syndrome.
10.1007/s11684-017-0575-y
- Author:
Qiaohong LAI
1
;
Wenpei XIANG
2
;
Qing LI
3
;
Hanwang ZHANG
3
;
Yufeng LI
3
;
Guijin ZHU
3
;
Chengliang XIONG
2
;
Lei JIN
4
Author Information
1. Reproductive Medicine Center, Tongji Hospital, Huazhong University of Science and Technology, Wuhan, 430030, China. lqh74@126.com.
2. Family Planning Research Institute, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.
3. Reproductive Medicine Center, Tongji Hospital, Huazhong University of Science and Technology, Wuhan, 430030, China.
4. Reproductive Medicine Center, Tongji Hospital, Huazhong University of Science and Technology, Wuhan, 430030, China. ljin@tjh.tjmu.edu.cn.
- Publication Type:Journal Article
- Keywords:
IVF-ET;
NADPH oxidase;
PCOS;
ROS;
granulosa cell
- MeSH:
Abortion, Spontaneous;
epidemiology;
Acetophenones;
therapeutic use;
Adult;
Apoptosis;
drug effects;
Embryo Transfer;
Female;
Fertilization in Vitro;
Granulosa Cells;
metabolism;
Humans;
NADPH Oxidases;
antagonists & inhibitors;
Onium Compounds;
therapeutic use;
Oocyte Retrieval;
Oxidative Stress;
Polycystic Ovary Syndrome;
drug therapy;
Pregnancy;
Pregnancy Rate;
Reactive Oxygen Species;
metabolism
- From:
Frontiers of Medicine
2018;12(5):518-524
- CountryChina
- Language:English
-
Abstract:
The increased levels of intracellular reactive oxygen species (ROS) in granulosa cells (GCs) may affect the pregnancy results in women with polycystic ovary syndrome (PCOS). In this study, we compared the in vitro fertilization and embryo transfer (IVF-ET) results of 22 patients with PCOS and 25 patients with tubal factor infertility and detected the ROS levels in the GCs of these two groups. Results showed that the PCOS group had significantly larger follicles on the administration day for human chorionic gonadotropin than the tubal factor group (P < 0.05); however, the number of retrieved oocytes was not significantly different between the two groups (P > 0.05). PCOS group had slightly lower fertilization, cleavage, grade I/II embryo, clinical pregnancy, and implantation rates and higher miscarriage rate than the tubal factor group (P > 0.05). We further found a significantly higher ROS level of GCs in the PCOS group than in the tubal factor group (P < 0.05). The increased ROS levels in GCs caused GC apoptosis, whereas NADPH oxidase 2 (NOX2) specific inhibitors (diphenyleneiodonium and apocynin) significantly reduced the ROS production in the PCOS group. In conclusion, the increased ROS expression levels in PCOS GCs greatly induced cell apoptosis, which further affected the oocyte quality and reduced the positive IVF-ET pregnancy results of women with PCOS. NADPH oxidase pathway may be involved in the mechanism of ROS production in GCs of women with PCOS.
