Preparation and evaluation of carboxymethyl chitosan/sodium alginate hydrogel for cartilage tissue engineering.
- Author:
Yun-Jie LI
1
;
Bin-Hong TENG
1
;
Yan-Hong ZHAO
1
;
Qiang YANG
2
;
Lian-Yong WANG
3
;
Ying HUANG
1
Author Information
- Publication Type:Journal Article
- Keywords: carboxymethyl chitosan; cartilage tissue engineering; hydrogel; sodium alginate; umbilical cord mesenchymalstem cell
- MeSH: Alginates; Cartilage; Chitosan; Hydrogels; Tissue Engineering
- From: West China Journal of Stomatology 2019;37(3):253-259
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:This study aimed to optimize the preparation of carboxymethyl chitosan/sodium alginate (CMCS/OSA) compound hydrogels. This study also aimed to investigate the applicability of the hydrogels in cartilage tissue engi-neering.
METHODS:Three groups of CMCS/OSA composite hydrogels with amino-to-aldehyde ratios of 2∶1, 1∶1 and 1∶2 were prepared. The microstructure, physical properties, and cell biocompatibility of the three groups of CMCS/OSA com-posite hydrogels were evaluated. Samples were subjected to scanning electron microscopy, rheological test, adhesion tension test, swelling rate test, and cell experiments to identify the CMCS/OSA composite hydrogel with the cross-linking degree that can meet the requirements for scaffolds in cartilage tissue engineering.
RESULTS:The experimental results showed that the CMCS/OSA hydrogel with a amine-to-aldhyde ratio of 1∶1 had good porosity, suitable gelling time, strong adhesive force, stable swelling rate, and good cellular biocompatibility.
CONCLUSIONS:The CMCS/OSA compound hydrogel prepared with a 1∶1 ratio of amino and aldehyde groups has potential applications in cartilage tissue engineering.
