Effects of staurosporine on the contraction of self-assembled constructs of goat temporomandibular joint disc cells.

Shan-ying Bao; Guang-jie Bao; Yu-yao Tang; Lin Liu; Hong Kang

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Effects of staurosporine on the contraction of self-assembled constructs of goat temporomandibular joint disc cells.

Author: Shan-Ying BAO ¹ ; Guang-Jie BAO ^{2
,

3} ; Yu-Yao TANG ¹ ; Lin LIU ^{2
,

3} ; Hong KANG ¹
Author Information

1. Institute of Stomatology, Lanzhou University, Lanzhou 730000, China.
2. Key Laboratory of Stomatology of State Ethnic Affairs Commission, Northwest Minzu University, Lanzhou 730030, China
3. Key Laboratory of Oral Diseases of Gansu Province, Northwest Minzu University, Lanzhou 730030, China.
Publication Type:Journal Article
Keywords: self-assembled construct; staurosporine; temporomandibular joint disc
MeSH: Animals; Collagen Type I; Glycosaminoglycans; Goats; Staurosporine; pharmacology; Temporomandibular Joint; Temporomandibular Joint Disc; cytology; drug effects
From: West China Journal of Stomatology 2019;37(3):275-279
CountryChina
Language:Chinese
Abstract: OBJECTIVE:The effects of the staurosporine on contraction of self-assembled constructs and extracellular matrix syntheses of goat temporomandibular joint discs were investigated.
METHODS:Goat temporomandibular joint disc cells were isolated and cultured to P3, and 5.5×10⁶ cells were combined with different concentrations of staurosporine (0, 0.1, 1, 10, 100 nmol·L⁻¹) in agarose wells and cultured for one week. The samples were frozen and sectioned. Safranin-O, Picro-sirius red and immunohistochemical staining were performed to observe the distributions of the extracellular matrix and the expression of alpha-smooth muscle actin (α-SMA). Enzyme linked immunosorbent assay (ELISA) and Blyscan kits were utilized to quan--titatively detect the contents of type Ⅰ collagen (ColⅠ) and glycosaminoglycans (GAGs).
RESULTS:Each group of goat temporo-mandibular joint disc cells in the agarose wells were gathered to self-assemble into a disc-shaped base for 4 hours and then to gradually contract into a round shape. The Picro-sirius red staining was strong and indicated collagen distribution. The Safranin-O staining observed GAGs throughout the entire construct. The expression of ColⅠ was strongly posi-tive in the staurosporine groups; however, the expression of α-SMA was weak. ColⅠ and GAGs contents in the stau-rosporine groups were greater than that of the control group, especially in the 10 nmol·L⁻¹ group (P<0.01).
CONCLUSIONS:Staurosporine has a certain effect on the shrinkage of self-assembled constructs; however, such effect is not prominent. Staurosporine contributes to the construction synthesis of extracellular matrix.