Oral microbiological diversity in patients with salivary adenoid cystic carcinoma.
- Author:
Xing LIU
1
,
2
,
3
;
Qi-Fen YANG
1
,
2
,
3
;
Ning GAN
1
,
2
,
3
;
De-Qin YANG
1
,
2
,
3
Author Information
- Publication Type:Journal Article
- Keywords: high-throughput sequencing; microbial diversity; saliva; salivary adenoid cystic carcinoma
- MeSH: Bacteria; isolation & purification; Carcinoma, Adenoid Cystic; microbiology; Humans; Porphyromonas; RNA, Ribosomal, 16S; Saliva; Salivary Gland Neoplasms; microbiology
- From: West China Journal of Stomatology 2019;37(3):304-308
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:The aim of this study was to identify the differences in microbial diversity and community in patients with salivary adenoid cystic carcinoma (SACC).
METHODS:Saliva was collected from 13 patients with SACC confirmed by histopathological diagnosis and 10 healthy control subjects. Total metagenomic DNA was extracted. The DNA amplicons of the V3-V4 hypervariable regions of the 16S rRNA gene were generated and subjected to high-throughput sequencing. Microbial diversity and community structure were analyzed with Mothur software.
RESULTS:A total of 16 genera of dominant bacteria in the SACC group were found, including Streptococcus (36.68%), Neisseria (8.55%), Prevotella_7 (7.53%), and Veillonella (6.37%), whereas 15 dominant bacteria in the control group were found, including Streptococcus (18.41%), Neisseria (18.20%), Prevotella_7 (8.89%), Porphyromonas (6.20%), Fusobacterium (5.86%) and Veillonella (5.82%). The statistically different phyla between the two groups were Firmicutes, Proteobacteria and Fusobacterium (P<0.05). The statistically different genera between the two groups were Streptococcus, Neisseria and Porphyromonas (P<0.05), and Capnocytophaga was only detected in patients with SACC.
CONCLUSIONS:Significant differences were observed in the oral microorganisms between the two groups.
