- Author:
Hong ZHANG
1
;
Li-Na WANG
1
;
Mei-Na ZUO
1
;
Ming DONG
1
;
Dong-Mei SHI
1
;
Hui-Jun XU
1
;
Wei-Dong NIU
1
Author Information
- Publication Type:Journal Article
- Keywords: Bruton’s tyrosine kinase; bone destruction; osteoclast; small interfering RNA transfection
- MeSH: Agammaglobulinaemia Tyrosine Kinase; Cell Differentiation; Cell Proliferation; Macrophages; Osteoclasts; RANK Ligand
- From: West China Journal of Stomatology 2019;37(4):361-365
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To observe the effect of Bruton's tyrosine kinase (BTK) on the proliferation and differentiation of osteoclasts and to explore the mechanism of BTK on bone destruction in periapical periodontitis.
METHODS:After RAW264.7 cells induced with 100 ng·L⁻¹ receptor activator for nuclear factor-κB ligand (RANKL) for 5 days, osteoclast induction was confirmed by light microscopy, tartrate-resistant acid phosphatase (TRAP) staining, and quantitative real-time PCR (RT-qPCR). Then, BTK-small interfering RNA (BTK-siRNA) was transfected into cells induced for 5 days. After 24 h, the expression of TRAP mRNA was measured using RT-qPCR, and the proliferation and differentiation of osteoclasts were detected using CCK-8 and TRAP activity assay. Statistical analysis was performed.
RESULTS:After RAW264.7 was induced with RANKL for 5 days, a large number of round, ellipse, irregularly protuberant, and TRAP-positive macrophages were observed under light microscopy. The expression of TRAP mRNA significantly reduced after 24 h of BTK-siRNA transfection (P<0.05). The detection of CCK-8 and TRAP activities showed that the proliferation and differentiation of osteoclasts significantly decreased (P<0.05).
CONCLUSIONS:Silencing of BTK can inhibit the proliferation and differentiation of osteoclasts. BTK can be used as a new target for the inhibition of osteoclasts.