Effect of long chain non-coding RNA H19 on the migration and invasion of oral cancer cells and its molecular mechanism.

Jun-Fang ZHAO; Zhi-An ZHA; Wei-Hong XIE; Hai-Bin WANG; Xin-Ming LI; Qiang SUN; Ming-Lei SUN

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Effect of long chain non-coding RNA H19 on the migration and invasion of oral cancer cells and its molecular mechanism.

Author: Jun-Fang ZHAO ¹ ; Zhi-An ZHA ¹ ; Wei-Hong XIE ¹ ; Hai-Bin WANG ¹ ; Xin-Ming LI ¹ ; Qiang SUN ¹ ; Ming-Lei SUN ¹
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1. Dept. of Oral and Maxillofacial Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.
Publication Type:Journal Article
Keywords: invasion; long chain non-coding RNA H19; migration; oral cancer
MeSH: Cell Line, Tumor; Gene Expression Regulation, Neoplastic; Genes, Tumor Suppressor; Humans; MicroRNAs; Mouth Neoplasms; RNA, Long Noncoding
From: West China Journal of Stomatology 2019;37(4):378-383
CountryChina
Language:Chinese
Abstract: OBJECTIVE:To investigate the effect of the long chain non-coding RNA H19 (lncRNA H19) on the invasion and migration of oral cancer cells and its related molecular mechanism.
METHODS:The expression levels of lncRNA H19, miR-107, and cyclin-dependent kinase 6 (CDK6) in the immortalized oral epithelial cell line HIOEC and the oral cancer cell line CAL27 were detected by real-time quantitative polymerase chain reaction. CAL27 cells were transfected with siRNA H19, miR-107 mimics, pcDNA H19, or anti-miR-107, and the effects of H19 and miR-107 on the invasion and migration of cells were examined via Transwell assay. The TargetScan database predicted the targeting of H19, miR-107, and CDK6. Double luciferase reporter gene assay was performed to detect interactions among H19, miR-107, and CDK6. Western blot analysis was conducted to examine the effects of H19 and miR-107 on the protein level of the target gene CDK6.
RESULTS:Compared with that in HIOEC cells, the expression of H19 was significantly increased in CAL27 cells (P<0.05). After transfection with siRNA H19, the expression of H19 decreased, and the invasion and migration ability of CAL27 cells were inhibited (P<0.05). H19 could bind specifically to the 3'-UTR of miR-107 to modulate the expression of miR-107. Compared with that in HIOEC cells, the expression of miR-107 significantly decreased in CAL27 cells (P<0.05). The expression of miR-107 increased after transfection with siRNA H19, and anti-mir-107 co-transfection could promote the invasion and migration ability of siRNA H19 in CAL27 cells (P<0.05). Compared with that in HIOEC cells, CDK6 expression significantly increased in CAL27 cells (P<0.05), and the expression level of the gene was coregulated by H19 and miR-107 (P<0.05).
CONCLUSIONS:lncRNA H19 plays an important role in the development of oral cancer. It can regulate the invasion and migration of oral cancer cells by targeting the miR-107/CDK6 signaling axis.