Effects of cysteinyl leukotriene receptors on phagocytosis of mouse microglial cells.
- Author:
Xiaorong WANG
1
;
Yunbi LU
2
;
Weiping ZHANG
2
;
Erqing WEI
2
;
Sanhua FANG
2
Author Information
1. Department of Pharmacy, Women's Hospital, Zhejiang University School of Medicine, Hangzhou 310006, China.
2. Department of Pharmacology, Zhejiang University School of Medicine, Hangzhou 310058, China.
- Publication Type:Journal Article
- MeSH:
Acetates;
pharmacology;
Animals;
Cell Line;
Cyclohexanecarboxylic Acids;
pharmacology;
Lipopolysaccharides;
pharmacology;
Mice;
Microglia;
cytology;
Phagocytosis;
drug effects;
Phthalic Acids;
pharmacology;
Protein Binding;
drug effects;
Quinolines;
pharmacology;
Receptors, Leukotriene;
agonists;
metabolism
- From:
Journal of Zhejiang University. Medical sciences
2018;47(1):10-18
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:: To determine the effects of cysteinyl leukotriene receptors (CysLTR and CysLTR) on phagocytosis of mouse BV2 microglial cells.
METHODS:: BV2 cells were stimulated with microglial activators lipopolysaccharide (LPS) or CysLT receptor agonists LTD. The phagocytosis of BV2 cells was observed by immunofluorescence analysis and flow cytometry. The intracellular distributions of CysLTR and CysLTR in BV2 cells were examined with immunofluorescence staining.
RESULTS:: Both LPS and LTD could significantly enhance the phagocytosis of BV2 cells, and such effect could be inhibited by CysLTR selective antagonist Montelukast and CysLTR selective antagonist HAMI 3379. The activation of BV2 cells induced by LTD or LPS resulted in changes in intracellular distributions of CysLTR and CysLTR. CysLTR and CysLTR was co-localization with a similar distribution.
CONCLUSIONS:: CysLTR and CysLTR regulate the phagocytosis of mouse BV2 microglial cells with a synergistic effect.
