Chlorogenic acid inhibits non-enzymatic glycation and oxidation of low density lipoprotein.
- Author:
Rui CAI
1
;
Shuqing CHEN
1
;
Shenhua JIANG
2
Author Information
1. College of Pharmacy, Zhejiang University, Hangzhou 310058, China.
2. School of Pharmacy and Life Science, Jiujiang University, Jiujiang 332005, Jiangxi Province, China.
- Publication Type:Journal Article
- MeSH:
Chlorogenic Acid;
pharmacology;
Glycosylation;
drug effects;
Lipoproteins, LDL;
metabolism;
Oxidation-Reduction;
drug effects;
Thiobarbituric Acid Reactive Substances;
analysis
- From:
Journal of Zhejiang University. Medical sciences
2018;47(1):27-34
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:: To investigate the effect of chlorogenic acid (CGA) on non-enzymatic glycation and oxidation of low density lipoprotein (LDL).
METHODS:: The non-enzymatic glycation incubation system of LDL-glucose was established. The contents of early glycation products (Amodori product) and intermediate products (dicarbonyl compound) were determined by ultraviolet-visible spectrophotometry, and the content of advanced glycation end products (AGEs) was determined by fluorescence spectrophotometry. The LDL oxidation incubation system was established. The contents of thiobarbituric acid reactive substances(TBARS) and conjugated diene were determined by ultraviolet-visible spectrophotometry. The tryptophan fluorescence quenching, and the content of lipofuscin, total fluorescence products, active aldehydes and malondialdehyde were determined by fluorescence spectrophotometry, and further verified by three-dimensional fluorescence spectroscopy.
RESULTS:: In the LDL glycation experiment, 150 μg/mL and 300 μg/mL CGA inhibited the formation of Amadori product, dicarbonyl compounds and AGEs. In the LDL oxidation experiment, 15 μg/mL and 25 μg/mL CGA inhibited the formation of TBARS effectively; 5 μg/mL and 10 μg/mL CGA inhibited tryptophan fluorescence quenching, and the formation of active aldehydes, malondialdehyde, total fluorescence products, lipofuscin and conjugated diolefine. And the three-dimensional fluorescence spectroscopy showed the same results.
CONCLUSIONS:: CGA can inhibit non-enzymatic glycation and oxidation of LDL.
