TLR/NF-κB independent signaling pathway in TNF-α suppression of diabetic MyD88-knockout mice after polysaccharides administration.
- Author:
Tingting LIU
1
;
Lingxiao WANG
1
;
Xiaohui YANG
1
;
Zhiqing YAO
1
;
Huizhen CAI
1
Author Information
1. School of Public Health and Management, Ningxia Medical University, Yinchuan 750004, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Diabetes Mellitus, Experimental;
Drugs, Chinese Herbal;
pharmacology;
Gene Expression Regulation;
drug effects;
Macrophages, Peritoneal;
drug effects;
Mice;
Mice, Knockout;
Myeloid Differentiation Factor 88;
genetics;
NF-kappa B;
genetics;
Random Allocation;
Signal Transduction;
drug effects;
Tumor Necrosis Factor-alpha;
blood;
metabolism
- From:
Journal of Zhejiang University. Medical sciences
2018;47(1):35-40
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:: To investigate the effect of polysaccharides (LBPs) on TLR/NF-κB independent pathway and serum tumor necrosis factor (TNF-α) level in diabetic MyD88-knockout mice.
METHODS:: Diabetes was induced by feeding high-fat/high-sugar diet and injection of low-dose streptozotocin in MyD88-knockout mice. The diabetic mice were randomly divided into model group, positive control group and LBPs group. The expressions of TRAM, TRIF, TRAF6, RIP1 and TNF-α mRNA and proteins in mouse peritoneal macrophages were detected by real-time RT-PCR and Western blotting after LBPs treatment for 3 month. Serum TNF-α was determined with ELISA kit.
RESULTS:: Real time RT-PCR showed that compared with model group, the relative expressions of and mRNA in macrophages of LBPs group were significantly decreased and expression of was significantly increased (all <0.05). Expression of TRAM, TRIF, TRAF6, RIP1 and TNF-α proteins as well as serum TNF-α level had no significant difference between LBPs group and model group (all >0.05).
CONCLUSIONS:: LBPs may not inhibit serum TNF-α level through TLR/NF-κB independent pathway.
