Design of Dyson Flow Cytometry System.
10.3969/j.issn.1671-7104.2019.04.010
- Author:
Xiaoliang ZHANG
1
;
Bidou WANG
2
;
Gangyin LUO
2
Author Information
1. School of Biomedical Engineering, University of Science and Technology of China, Hefei, 230026.
2. Suzhou Institute of Biomedical Engineer and Technology, Chinese Academy of Science, Suzhou, 215163.
- Publication Type:Journal Article
- Keywords:
Dyson light splitting;
electron multiplying charge coupled device;
flow cytometer;
spectral analysis
- MeSH:
Equipment Design;
Flow Cytometry;
instrumentation
- From:
Chinese Journal of Medical Instrumentation
2019;43(4):270-274
- CountryChina
- Language:Chinese
-
Abstract:
In order to meet the needs of the flow cytometry for the simultaneous analysis of multiple fluorescence wavelengths and small volume, the design method of flow cytometry spectrum analysis system is presented by analyzing the characteristics of Dyson structure. And according to the method, a flow cytometry spectrum analysis system is disigned with Dyson type.The system's spectral range is 400 nm to 800 nm, the defocused spot size is less than the pixel size 24μ mm, the ransfer function value is above 0.8 at the Nyquist cut-off frequency 21 lp/mm,the spectral resolution is less than 3 nm, and the overall size is 83.54 mm×85.60 mm.The system has good optical performance and small volume, which meets the needs of the flow cytometry fluorescence spectral analysis. The outstanding innovation of this system is the application of Dyson light splitting structure and EMCCD detector which is high speed and high sensitivity.
