Saliva of periodontitis patients promotes macrophage differentiation and activation.
- Author:
Xiao-Ying LI
1
;
Qian LI
1
;
Bo-Miao CUI
1
;
Li-Wei WANG
1
;
Ya-Ping FAN
1
;
Jiao CHEN
1
;
Yun FENG
1
;
Ping ZHANG
1
Author Information
- Publication Type:Journal Article
- Keywords: chronic periodontitis; macrophage; saliva
- MeSH: Cell Differentiation; Humans; Leukocytes, Mononuclear; Macrophages; Monocytes; Periodontitis; immunology; Saliva
- From: West China Journal of Stomatology 2018;36(5):508-513
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:The aim of this study was to investigate the effect of saliva of patients with chronic periodontitis (CPD) on the differentiation, activation, and secretion of osteoclast-maturing mediators of macrophages.
METHODS:A total of 40 saliva samples were collected from healthy donors (n=20) and severe periodontitis patients (n=20). Peripheral blood mononuclear cells (PBMCs) and THP-1 monocyte line cells were challenged with 15% saliva for 5 days. The phenotype, surface marker, and phagocytosis of macrophages were analyzed by flow cytometry and microscopy. Osteoclast-maturing mediators were assayed by using enzyme-linked immunosorbent assay (ELISA) kits.
RESULTS:When PBMCs were treated with CPD saliva for 5 days, 61.25%±11.33% of cells were transformed into large granular cells; 86.78%±13.69% of large granular cells were identified as CD14⁺⁺CD16⁺ macrophages. When THP-1 cells were treated with CPD saliva, most cells attached to the bottom of cell culture plates, thereby exhibiting macrophage morphology and releasing additional osteoclast-maturing mediators. Furthermore, the phagocytosis of THP-1 cells considerably increased in the presence of CPD saliva (66.35%±9.67%) compared with medium control (33.33%±7.52%), or healthy saliva (40.71%±3.52%).
CONCLUSIONS:Saliva from patients with CPD can induce macrophage differentiation, activate phagocytose microorganisms, and secrete osteoclast-maturing mediators.
