Effects of calcium ion on the migration and osteogenic differentiation of human osteoblasts.
- Author:
Qun LEI
1
;
Dong LIN
1
;
Wen-Xiu HUANG
1
;
Dong WU
1
;
Jiang CHEN
1
Author Information
- Publication Type:Journal Article
- Keywords: bone substitute; calcium ion; calcium sensitive receptor; human osteoblast; migration
- MeSH: Calcium; physiology; Cell Differentiation; Cell Movement; Cell Proliferation; Humans; Osteoblasts; Osteogenesis; physiology; Signal Transduction
- From: West China Journal of Stomatology 2018;36(6):602-608
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:This study aimed to investigate the effect of calcium ion (Ca²⁺) on the migration and osteogenic differentiation of human osteoblasts and explore the proper concentration and correlation mechanism.
METHODS:A series of Ca²⁺ solutions with different concentrations was prepared. Osteoblast migration was assessed by Transwell assay, and proliferation was studied via the CCK-8 colorimetric assay. The mRNA expression of osteogenic genes was examined via reverse transcription-polymerase chain reaction (RT-PCR), and the mineralized nodule was examined by alizarin red-S method. After calcium sensitive receptor (CaSR) antagonism, Ca²⁺-induced migration and osteogenic differentiation were analyzed.
RESULTS:In the migration experiment, 2, 4, and 6 mmol·L⁻¹ Ca²⁺ could promoted osteoblast migration at three timepoints (8, 16, and 24 h), whereas 10 mmol·L⁻¹ Ca²⁺ considerably inhibited migration at 8 h. The Ca²⁺ concentration range of 2-10 mmol·L⁻¹ could promote proliferation, osteogenic differentiation, and mineralization of human osteoblasts. Moreover, mineralization was predominantly induced by 8 and 10 mmol·L⁻¹ Ca²⁺. CaSR antagonism could reduce Ca²⁺-induced migration and osteogenic differentiation of human osteoblasts.
CONCLUSIONS:Low Ca²⁺ concentration favored osteoblast migration, whereas high Ca²⁺ concentration favored osteogenic differentiation. The Ca²⁺ concentrations of 4 and 6 mmol·L⁻¹ could substantially induce osteoblast migration and osteogenic differentiation, and the Ca²⁺-CaSR pathway participated in signal transduction.
