Effects of large tumor suppressor homolog 2 gene overexpression on the proliferation and apoptosis of oral squamous cell carcinoma.
- Author:
Zeng-Wen YUE
1
;
Shu-Bin WANG
1
;
Jin-Zhong LIU
1
Author Information
- Publication Type:Journal Article
- Keywords: apoptosis; large tumor suppressor homolog 2; oral squamous cell carcinoma; proliferation
- MeSH: Apoptosis; Carcinoma, Squamous Cell; genetics; metabolism; Cell Line, Tumor; Cell Proliferation; Gene Expression Regulation, Neoplastic; Humans; Mouth Neoplasms; genetics; metabolism; Protein-Serine-Threonine Kinases; physiology; Tumor Suppressor Proteins; physiology
- From: West China Journal of Stomatology 2018;36(6):609-612
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effect of large tumor suppressor homolog 2 (LATS2) gene overexpression on the proliferation and apoptosis of oral squamous cell carcinoma (OSCC).
METHODS:Lentivirous particles were transferred into SCC-25 cell to upregulate LATS2 gene expression. Cell proliferation was detected by CCK-8 assay. Apoptosis was detected through flow cytometry. The expression changes of Bax, Bcl-2, and LATS2 were analyzed by Western blot.
RESULTS:Gene transfection increased LATS2 expression. Compared with the control group and pEGFP-control group, SCC-25 cell proliferation in the pGFP-LATS2 group was inhibited, whereas the apoptosis ratio increased (P<0.05). Bcl-2 expression decreased, and Bax expression increased.
CONCLUSIONS:Overexpression of LATS2 could inhibit SCC-25 cell proliferation and induce apoptosis.
