Expression of Acetaldehyde Dehydrogenase in Gefitinib-resistant Human Lung Adenocarcinoma HCC-827/GR Cells.
10.3779/j.issn.1009-3419.2018.06.01
- Author:
Tingting YANG
1
;
Jingjing GU
2
;
Ting LIU
1
;
Haibin MA
1
;
Xiaona MA
1
;
Jin TAO
1
;
Yiran JIN
1
;
Xueyun LIANG
1
Author Information
1. Institute of Human Stem Cell Research, General Hospital of Ningxia Medical University,
Yinchuan 750004, China.
2. College of Clinical Medicine, General Hospital of Ningxia Medical University,
Yinchuan 750004, China.
- Publication Type:Journal Article
- Keywords:
Acetaldehyde dehydrogenase;
Gefitinib;
HCC-827;
Human lung adenocarcinoma
- MeSH:
Adenocarcinoma;
pathology;
Adenocarcinoma of Lung;
Aldehyde Oxidoreductases;
antagonists & inhibitors;
genetics;
Cell Line, Tumor;
Drug Resistance, Neoplasm;
genetics;
Enzyme Inhibitors;
pharmacology;
Gefitinib;
Gene Expression Regulation, Neoplastic;
drug effects;
Humans;
Lung Neoplasms;
pathology;
Quinazolines;
pharmacology
- From:
Chinese Journal of Lung Cancer
2018;21(6):431-436
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Tumor recurrence and drug resistance are the main causes of death in tumor patients. The family of acetaldehyde dehydrogenase (ALDH) is closely related to the proliferation, migration, invasion and resistance of tumor cells, and different ALDH subtypes are expressed in different tumor cells. The aim of this study is to elucidate the ALDH subtype in human lung adenocarcinoma HCC-827/GR cells, which resistant to the gefitinib.
METHODS:The human lung adenocarcinoma HCC-827 cells were used to generate the gefitinib-resistant HCC-827/GR cells; the expression of ALDH subtype in either HCC-827 or HCC-827/GR was detected by flow cytometry; The proliferative capacity and sensitivity to gefitinib of hcc-827/GR cells were analyzed by MTT assay before and after treatment with 100 μmol/L diethyllaminaldehyde (DEAB); Real-time quantitative PCR was used to detect the expression of ALDH subtypes at mRNA levels in hcc-827 cells and hcc-827/GR cells.
RESULTS:Compared with HCC-827 cells, the positive rate of ALDH in HCC-827/GR cells increased. The proliferation ability of HCC-827/GR cells decreased after treatment with 100 μmol/L DEAB. Compared with HCC-827 cells, the expression of ALDH1A1 and ALDH1L1 mRNA was increased in hcc-827/GR cells, but the ALDH3B2 expression was decreased.
CONCLUSIONS:ALDH might be used as a molecular biomarker to test the gefitinib-resistant to lung adenocarcinoma cancer cells, and the ALDH1A1 may play a role in gefitinib resistance in lung cancer.
