Metabolomics Study on the Differences of Endogenous Small Molecule
between A549/DDP and A549 Cells Based on High Solution UPLC-TOF-MS.
10.3779/j.issn.1009-3419.2018.08.01
- Author:
Weipeng HONG
1
;
Yonghua ZHAO
2
;
Lin CAO
1
;
Di CAO
3
;
Zhongxiang ZHAO
3
;
Jing JIN
1
Author Information
1. School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, China.
2. Clinical Lab, People's Hospital of Nanzhang County, Nanzhang 441500, China.
3. School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou 510006, China.
- Publication Type:Journal Article
- Keywords:
Chemoresistance;
Cisplatin;
Lung neoplasms;
Metabolomics
- MeSH:
A549 Cells;
Carcinoma, Non-Small-Cell Lung;
pathology;
Chromatography, High Pressure Liquid;
Drug Resistance, Neoplasm;
Humans;
Lung Neoplasms;
pathology;
Mass Spectrometry;
Metabolomics;
methods
- From:
Chinese Journal of Lung Cancer
2018;21(8):571-577
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Cisplatin acquired resistance is a vital problem in the chemotherapy of non-small cell lung cancer, which needs to be further addressed. In recent years, obtaining drug resistant cells from cell cultivation and serving for metabolomics research to find differential metabolites and get potential biomarkers, is a good reference for clinical research and cancer treatment. This study aimed to obtain metabolite information related to cisplatin resistance through metabolomics analysis.
METHODS:Metabolites were extracted from A549 cells and cisplatin resistant A549/DDP cells, and ultraperformance liquid chromatography coupled with time of flight mass spectrometry was used to perform metabolomic analysis of endogenous molecules of the two cells and obtain metabolic differences.
RESULTS:Through data analysis, 40 metabolites were identified as differential metabolites, mainly involving phospholipids, fatty acids, amino acids and metabolites related to energy metabolism.
CONCLUSIONS:The drug resistance of A549/DDP cells may be caused by the changes of cell membrane structure and related metabolic pathways.
