Microwave Hyperthermia Combined with Gemcitabine Inhibits Proliferation
and Induces Apoptosis of Human Lung Squamous Carcinoma Cells.
10.3779/j.issn.1009-3419.2018.11.01
- Author:
Yang YANG
1
;
Yanyan ZHAO
2
;
Shenglin MA
2
;
Daoke YANG
1
Author Information
1. Department of Radiation Oncology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450002, China.
2. Affiliated Hangzhou First People's Hospital, Zhejiang University School of Medicine, Hangzhou 310006, China.
- Publication Type:Journal Article
- Keywords:
Apoptosis;
Gemcitabine;
Lung squamous cell carcinoma;
Microwave hyperthermia;
Proliferation
- MeSH:
Apoptosis;
drug effects;
radiation effects;
Carcinoma, Squamous Cell;
pathology;
Caspase 3;
metabolism;
Caspase 8;
metabolism;
Cell Line, Tumor;
Cell Proliferation;
drug effects;
radiation effects;
Combined Modality Therapy;
Deoxycytidine;
analogs & derivatives;
pharmacology;
Humans;
Hyperthermia, Induced;
Lung Neoplasms;
pathology;
Microwaves
- From:
Chinese Journal of Lung Cancer
2018;21(11):805-814
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Lung cancer is one of the highest morbidity and mortality in the world and it is very important to find an effective anti-tumor method. Microwave hyperthermia, a new treatment technology, has been getting more and more attention. This study was designed to investigate the effects of microwave hyperthermia combined with gemcitabine on the proliferation and apoptosis of human lung squamous cell carcinoma (NCI-H1703 and NCI-H2170) in vitro.
METHODS:The proliferation of cells treated with microwave hyperthermia, the effect of gemcitabine on cell proliferation and the proliferation of cells treated with different methods of microwave hyperthermia and gemcitabine were detected by CCK-8 assay. Colony formation assay was used to measure the colony formation of human lung squamous cell carcinoma cells. Flow cytometry assay was used to detect the total apoptosis rates of the treated cells. Caspase-3, Caspase-8 activity assay was used to detect the activity of Caspase-3, Caspase-8 enzyme in each group of cells. CCK-8 assay was used to detect the effect of control group, AC-DEVD (Caspase-3 inhibitor) group, thermalization combined group, and thermal AC-DEVD combined group on cell proliferation. The levels of p53, Caspase-3, Cleaved-Caspase-3, PARP, Bax and BCL-2 protein expression were detected using Western blot assay.
RESULTS:Our results demonstrated that microwave hyperthermia inhibited the proliferation of lung squamous cell carcinoma. The IC₅₀ values of gemcitabine for the two cells were 8.89 μmol/L and 44.18 μmol/L, respectively. The first chemotherapy after microwave hyperthermia has synergistic effect on the two lung squamous cell carcinoma cells and can significantly inhibit the cell clone formation (P<0.001), promote cell apoptosis (P<0.001) and increase Caspase-3 enzyme activity (P<0.001). However, it has no effect on Caspase-8 enzyme activity (P>0.05). Furthermore, Western blot analysis showed that microwave hyperthermia combined with gemcitabine could up-regulate the p53, Caspase-3, Cleaved-Caspase-3, Cleaved-PARP and Bax protein expression.
CONCLUSIONS:Microwave hyperthermia combined with gemcitabine remarkably inhibit the proliferation and induce apoptosis of human lung squamous cell carcinoma in vitro. This effect may be associated with the activation of p53, cleavage of PARP protein, and induced the Caspase-3 dependent apoptosis.
