Analysis of differential expression of tight junction proteins in cultured oral epithelial cells altered by Porphyromonas gingivalis, Porphyromonas gingivalis lipopolysaccharide, and extracellular adenosine triphosphate.

Wei GUO; Peng WANG; Zhong-Hao LIU; Ping YE

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Analysis of differential expression of tight junction proteins in cultured oral epithelial cells altered by Porphyromonas gingivalis, Porphyromonas gingivalis lipopolysaccharide, and extracellular adenosine triphosphate.

Author: Wei GUO ¹ ; Peng WANG ² ; Zhong-Hao LIU ³ ; Ping YE ⁴
Author Information

1. Department of Endodontics, Yantai Stomatological Hospital, Binzhou Medical University, Yantai, China.
2. Department of Pediatrics, Yantai Stomatological Hospital, Binzhou Medical University, Yantai, China.
3. Department of Implant, Yantai Stomatological Hospital, Binzhou Medical University, Yantai, China.
4. Institute of Dental Research, Centre for Oral Health, Westmead Hospital, Westmead, Australia.
Publication Type:Journal Article
MeSH: Adenosine Triphosphate; pharmacology; Cells, Cultured; Epithelial Cells; cytology; Gene Expression; Humans; Immunoblotting; Lipopolysaccharides; pharmacology; Mouth Mucosa; cytology; Porphyromonas gingivalis; immunology; Real-Time Polymerase Chain Reaction; Tight Junction Proteins; metabolism; Up-Regulation
From: International Journal of Oral Science 2018;10(1):e8-e8
CountryChina
Language:English
Abstract: Tight junctions (TJs) are the most apical intercellular junctions of epithelial cells formed by occludin, claudins, junctional adhesion molecules (JAMs), and zonula occludens (ZO). Tight junction proteins can sense the presence of bacteria and regulate the transcription of target genes that encode effectors and regulators of the immune response. The aim of this study was to determine the impact of TJ proteins in response to Porphyromonas gingivalis (P. gingivalis), P. gingivalis lipopolysaccharide (P. gingivalis LPS), and extracellular adenosine triphosphate (ATP) in the oral epithelial cell culture model. Quantified real time-polymerase chain reaction (RT-PCR), immunoblots, and immunostaining were performed to assess the gene and protein expression in TJs. It was found that P. gingivalis infection led to transient upregulation of the genes encoding occludin, claudin-1, and claudin-4 but not JAM-A, claudin-15, or ZO-1, while P. gingivalis LPS increased claudin-1, claudin-15, and ZO-1 and decreased occludin, JAM-A, and claudin-4. Tight junction proteins showed significant upregulation in the above two groups when cells were pretreated with ATP for 3 h. The findings indicated that P. gingivalis induced the host defence responses at an early stage. P. gingivalis LPS exerted a more powerful stimulatory effect on the disruption of the epithelial barrier than P. gingivalis. ATP stimulation enhanced the reaction of TJ proteins to P. gingivalis invasion and LPS destruction of the epithelium.International Journal of Oral Science (2018) 10, e8; doi:10.1038/ijos.2017.51; published online 10 January 2018.