Streptococcus mutans activates the AIM2, NLRP3 and NLRC4 inflammasomes in human THP-1 macrophages.
10.1038/s41368-018-0024-z
- Author:
Yuri SONG
1
;
Hee Sam NA
1
;
Eunjoo PARK
1
;
Mi Hee PARK
1
;
Hyun Ah LEE
1
;
Jin CHUNG
2
Author Information
1. Department of Oral Microbiology, School of Dentistry, Pusan National University, Yangsan, 50162, Korea.
2. Department of Oral Microbiology, School of Dentistry, Pusan National University, Yangsan, 50162, Korea. jchung@pusan.ac.kr.
- Publication Type:Journal Article
- MeSH:
Blotting, Western;
CARD Signaling Adaptor Proteins;
immunology;
Calcium-Binding Proteins;
immunology;
Caspase 1;
immunology;
DNA-Binding Proteins;
immunology;
Enzyme-Linked Immunosorbent Assay;
Humans;
Immunity, Innate;
Inflammasomes;
immunology;
Interleukin-1beta;
immunology;
Macrophages;
immunology;
NLR Family, Pyrin Domain-Containing 3 Protein;
immunology;
Signal Transduction;
Streptococcus mutans;
immunology;
Tumor Necrosis Factor-alpha;
immunology
- From:
International Journal of Oral Science
2018;10(3):23-23
- CountryChina
- Language:English
-
Abstract:
Streptococcus mutans (S. mutans), a major aetiologic agent of dental caries, is involved in systemic diseases, such as bacterial endocarditis, if it enters the bloodstream through temporary bacteraemia. Interleukin (IL)-1β, a proinflammatory cytokine, is related to the host defences against pathogens, and its synthesis, maturation, and secretion are tightly regulated by the activation of the inflammasome, an inflammatory signalling complex. This study examined the signalling mechanism of IL-1β secretion and the inflammasome pathway induced by S. mutans to explain the molecular mechanism through which systemic infection by oral streptococci can occur. After infection of THP-1 cells with S. mutans, the expression of inflammasome components was detected using various methods. S. mutans induced IL-1β secretion via caspase-1 activation, and S. mutans-induced IL-1β secretion required absent in melanoma (AIM2), NLR family pyrin domain-containing 3 (NLRP3) and NLR family CARD domain-containing 4 (NLRC4) inflammasome activation. In particular, the S. mutans-induced NLRP3 inflammasome was mediated by adenosine triphosphate (ATP) release, potassium depletion and lysosomal damage. Our study provides novel insight into the innate immune response against S. mutans infection.
