TGF-β2 downregulates osteogenesis under inflammatory conditions in dental follicle stem cells.
10.1038/s41368-018-0028-8
- Author:
Soyoun UM
1
;
Joo-Hee LEE
2
;
Byoung-Moo SEO
3
Author Information
1. Biotooth Engineering Lab, Dental Research Institute, Dental Regenerative Biotechnology, Department of Dental Science, School of Dentistry, Seoul National University, Seoul, Korea.
2. Biotooth Engineering Lab, Department of Oral and Maxillofacial Surgery and Craniomaxillofacial Life Science, Dental Research Institute, School of Dentistry, Seoul National University, Seoul, Korea.
3. Biotooth Engineering Lab, Department of Oral and Maxillofacial Surgery and Craniomaxillofacial Life Science, Dental Research Institute, School of Dentistry, Seoul National University, Seoul, Korea. seobm@snu.ac.kr.
- Publication Type:Journal Article
- MeSH:
Adolescent;
Alkaline Phosphatase;
metabolism;
Animals;
Cell Differentiation;
Cell Proliferation;
Cell Survival;
Cells, Cultured;
Dental Sac;
cytology;
metabolism;
Down-Regulation;
Enzyme-Linked Immunosorbent Assay;
Female;
Humans;
Immunohistochemistry;
Male;
Mass Spectrometry;
Mice;
Nitric Oxide;
metabolism;
Osteogenesis;
drug effects;
Polymerase Chain Reaction;
Staining and Labeling;
Stem Cells;
cytology;
metabolism;
Transforming Growth Factor beta2;
pharmacology;
Young Adult
- From:
International Journal of Oral Science
2018;10(3):29-29
- CountryChina
- Language:English
-
Abstract:
Bone formation is important for the reconstruction of bone-related structures in areas that have been damaged by inflammation. Inflammatory conditions such as those that occur in patients with rheumatoid arthritis, cystic fibrosis, and periodontitis have been shown to inhibit osteoblastic differentiation. This study focussed on dental follicle stem cells (DFSCs), which are found in developing tooth germ and participate in the reconstruction of alveolar bone and periodontal tissue in periodontal disease. After bacterial infection of inflamed dental tissue, the destruction of bone was observed. Currently, little is known about the relationship between the inflammatory environment and bone formation. Osteogenic differentiation of inflamed DFSCs resulted in decreased alkaline phosphatase (ALP) activity and alizarin red S staining compared to normal DFSCs. Additionally, in vivo transplantation of inflamed and normal DFSCs demonstrated severe impairment of osteogenesis by inflamed DFSCs. Protein profile analysis via liquid chromatography coupled with tandem mass spectrometry was performed to analyse the differences in protein expression in inflamed and normal tissue. Comparison of inflamed and normal DFSCs showed significant changes in the level of expression of transforming growth factor (TGF)-β2. Porphyromonas gingivalis (P.g.)-derived lipopolysaccharide (LPS) was used to create in vitro inflammatory conditions similar to periodontitis. The osteogenic differentiation of LPS-treated DFSCs was suppressed, and the cells displayed low levels of TGF-β1 and high levels of TGF-β2. DFSCs treated with TGF-β2 inhibitors showed significant increases in alizarin red S staining and ALP activity. TGF-β1 expression was also increased after inhibition of TGF-β2. By examining inflamed DFSCs and LPS-triggered DFSCs, these studies showed both clinically and experimentally that the increase in TGF-β2 levels that occurs under inflammatory conditions inhibits bone formation.
