Chronic inflammation deteriorates structure and function of collagen fibril in rat temporomandibular joint disc.
10.1038/s41368-018-0036-8
- Author:
Sheng-Jie CUI
1
;
Yu FU
1
;
Yan LIU
1
;
Xiao-Xing KOU
1
;
Jie-Ni ZHANG
1
;
Ye-Hua GAN
2
;
Yan-Heng ZHOU
3
;
Xue-Dong WANG
4
Author Information
1. Department of Orthodontics, Peking University School and Hospital of Stomatology, 22# Zhongguancun South Avenue, Haidian District, Beijing, China.
2. Center for Temporomandibular Disorders and Orofacial Pain, Peking University School and Hospital of Stomatology, 22# Zhongguancun South Avenue, Haidian District, Beijing, China.
3. Department of Orthodontics, Peking University School and Hospital of Stomatology, 22# Zhongguancun South Avenue, Haidian District, Beijing, China. yanhengzhou@vip.163.com.
4. Department of Orthodontics, Peking University School and Hospital of Stomatology, 22# Zhongguancun South Avenue, Haidian District, Beijing, China. xuedongwang2012@gmail.com.
- Publication Type:Journal Article
- MeSH:
Animals;
Collagen;
ultrastructure;
Female;
Fibrillar Collagens;
ultrastructure;
Freund's Adjuvant;
adverse effects;
Inflammation;
chemically induced;
metabolism;
pathology;
Injections, Intra-Articular;
Random Allocation;
Rats;
Rats, Sprague-Dawley;
Temporomandibular Joint;
Temporomandibular Joint Disc;
physiopathology;
ultrastructure;
Temporomandibular Joint Disorders;
physiopathology
- From:
International Journal of Oral Science
2019;11(1):2-2
- CountryChina
- Language:English
-
Abstract:
Collagen is the building component of temporomandibular joint (TMJ) discs and is often affected by inflammation in temporomandibular disorders. The macromechanical properties of collagen are deteriorated by chronic inflammation. However, the mechanism by which inflammation influences disc function remains unknown. The relationship between the ultrastructure and nanomechanical properties of collagen in inflamed discs should be clarified. Seven-week-old female Sprague-Dawley rats were randomly divided into two groups. Chronic TMJ inflammation was induced by intra-articular injection of complete Freund's adjuvant, and samples were harvested after 5 weeks. Picrosirius staining revealed multiple colours under polarized light, which represented alternative collagen bundles in inflamed discs. Using atomic force microscopy scanning, the magnitude of Young's modulus was reduced significantly accompanied with disordered collagen fibril arrangement with porous architecture of inflamed discs. Transmission electron microscopy scanning revealed a non-uniform distribution of collagen fibres, and oversized collagen fibrils were observed in inflamed discs. Fourier transform infrared microspectroscopy revealed a decrease in 1 338 cm/amide II area ratio of collagen in different regions. The peak positions of amide I and amide II bands were altered in inflamed discs, indicating collagen unfolding. Our results suggest that sustained inflammation deteriorates collagen structures, resulting in the deterioration of the ultrastructure and nanomechanical properties of rat TMJ discs.
