Oral bacteria colonize and compete with gut microbiota in gnotobiotic mice.
10.1038/s41368-018-0043-9
- Author:
Bolei LI
1
;
Yang GE
1
;
Lei CHENG
1
;
Benhua ZENG
2
;
Jinzhao YU
1
;
Xian PENG
1
;
Jianhua ZHAO
3
;
Wenxia LI
2
;
Biao REN
1
;
Mingyun LI
1
;
Hong WEI
4
;
Xuedong ZHOU
5
Author Information
1. State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases, Sichuan University, Chengdu, China.
2. Department of Laboratory Animal Science, College of Basic Medical Sciences, Third Military Medical University, Chongqing, China.
3. Shanghai Majorbio Bio-pharm Technology Co., Ltd, Shanghai, China.
4. Department of Laboratory Animal Science, College of Basic Medical Sciences, Third Military Medical University, Chongqing, China. weihong63528@163.com.
5. State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases, Sichuan University, Chengdu, China. zhouxd@scu.edu.cn.
- Publication Type:Journal Article
- MeSH:
Animals;
Bacteria;
Gastrointestinal Microbiome;
Germ-Free Life;
Humans;
Mice;
Microbiota;
RNA, Ribosomal, 16S
- From:
International Journal of Oral Science
2019;11(1):10-10
- CountryChina
- Language:English
-
Abstract:
The oral microbiota is associated with oral diseases and digestive systemic diseases. Nevertheless, the causal relationship between them has not been completely elucidated, and colonisation of the gut by oral bacteria is not clear due to the limitations of existing research models. The aim of this study was to develop a human oral microbiota-associated (HOMA) mouse model and to investigate the ecological invasion into the gut. By transplanting human saliva into germ-free (GF) mice, a HOMA mouse model was first constructed. 16S rRNA gene sequencing was used to reveal the biogeography of oral bacteria along the cephalocaudal axis of the digestive tract. In the HOMA mice, 84.78% of the detected genus-level taxa were specific to the donor. Principal component analysis (PCA) revealed that the donor oral microbiota clustered with those of the HOMA mice and were distinct from those of specific pathogen-free (SPF) mice. In HOMA mice, OTU counts decreased from the stomach and small intestine to the distal gut. The distal gut was dominated by Streptococcus, Veillonella, Haemophilus, Fusobacterium, Trichococcus and Actinomyces. HOMA mice and human microbiota-associated (HMA) mice along with the GF mice were then cohoused. Microbial communities of cohoused mice clustered together and were significantly separated from those of HOMA mice and HMA mice. The Source Tracker analysis and network analysis revealed more significant ecological invasion from oral bacteria in the small intestines, compared to the distal gut, of cohoused mice. In conclusion, a HOMA mouse model was successfully established. By overcoming the physical and microbial barrier, oral bacteria colonised the gut and profiled the gut microbiota, especially in the small intestine.
