Filamentation initiated by Cas2 and its association with the acquisition process in cells.

Lei Wang; Xin YU; Mengjie LI; Guiqin Sun; Lin Zou; Tiansheng LI; Linlin Hou; Yameng Guo; Danfeng Shen; Di QU; Xunjia Cheng; Li Chen

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Filamentation initiated by Cas2 and its association with the acquisition process in cells.

Author: Lei WANG ¹ ; Xin YU ¹ ; Mengjie LI ¹ ; Guiqin SUN ² ; Lin ZOU ¹ ; Tiansheng LI ¹ ; Linlin HOU ¹ ; Yameng GUO ¹ ; Danfeng SHEN ¹ ; Di QU ¹ ; Xunjia CHENG ¹ ; Li CHEN ³
Author Information

1. Department of Medical Microbiology, Key Laboratory of Medical Molecular Virology of Ministries of Education and Health, School of Basic Medical Sciences, Fudan University, 131 Dongan Road, Shanghai, China.
2. College of Medical Technology, Zhejiang Chinese Medical University, Hangzhou, China.
3. Department of Medical Microbiology, Key Laboratory of Medical Molecular Virology of Ministries of Education and Health, School of Basic Medical Sciences, Fudan University, 131 Dongan Road, Shanghai, China. lichen_bk@fudan.edu.cn.
Publication Type:Journal Article
From: International Journal of Oral Science 2019;11(3):29-29
CountryChina
Language:English
Abstract: Cas1-and-Cas2-mediated new spacer acquisition is an essential process for bacterial adaptive immunity. The process is critical for the ecology of the oral microflora and oral health. Although molecular mechanisms for spacer acquisition are known, it has never been established if this process is associated with the morphological changes of bacteria. In this study, we demonstrated a novel Cas2-induced filamentation phenotype in E. coli that was regulated by co-expression of the Cas1 protein. A 30 amino acid motif at the carboxyl terminus of Cas2 is necessary for this function. By imaging analysis, we provided evidence to argue that Cas-induced filamentation is a step coupled with new spacer acquisition during which filaments are characterised by polyploidy with asymmetric cell division. This work may open new opportunities to investigate the adaptive immune response and microbial balance for oral health.