Sodium 4-phenylbutyrate Attenuates High-fat Diet-induced Impaired Spermatogenesis.

Er Hui Wang; San Qiao Yao; Ling Tao; Jin Yan XI

Return

Sodium 4-phenylbutyrate Attenuates High-fat Diet-induced Impaired Spermatogenesis.

Author: Er Hui WANG ^{1
,

2} ; San Qiao YAO ³ ; Ling TAO ³ ; Jin Yan XI ³
Author Information

1. Department of Nutrition and Food Hygiene, College of Public Health, Xinxiang Medical University, Xinxiang 453003, Henan, China
2. Henan Collaborative Innovation Center of Molecular Diagnosis and Laboratory Medicine, Xinxiang Medical University, Xinxiang 453003, Henan, China.
3. Department of Nutrition and Food Hygiene, College of Public Health, Xinxiang Medical University, Xinxiang 453003, Henan, China.
Publication Type:Journal Article
Keywords: Male infertility; Obesity; Semen; Sodium 4-phenylbutyrate; Spermatogenesis; Testis
MeSH: Animals; Diet, High-Fat; adverse effects; Male; Phenylbutyrates; pharmacology; Rats, Sprague-Dawley; Semen Analysis; Spermatogenesis; drug effects; Testis; drug effects; pathology; Testosterone; blood
From: Biomedical and Environmental Sciences 2018;31(12):876-882
CountryChina
Language:English
Abstract: OBJECTIVE:To determine the mitigating effects of sodium 4-phenylbutyrate (4-PBA) on high-fat diet (HFD)-induced spermatogenesis dysfunction.
METHODS:Male rats (n = 30) were randomly divided into three groups: control, HFD, and 4-PBA (HFD +4-PBA). After 13 weeks, rats were euthanized. Testes and epididymis were harvested for further analysis. Sex hormones were detected, and hematoxylin and eosin staining was performed to examine the histological changes in the testes. Semen samples were collected to evaluate sperm quality. Spermatogenic cell apoptosis was detected by TUNEL assay.
RESULTS:Compared with the control group, the final body weight and body weight gain were significantly higher in HFD-fed rats, while the testicle/body weight ratios were lower (P < 0.05). In HFD-fed rats, obvious pathological changes in the testicular tissue were observed. Treatment with 4-PBA attenuated HFD-induced histological damage, ameliorated the HFD-induced decrease in serum testosterone (T), and reduced the rate of testicular cell apoptosis (P < 0.05) in obese male rats. Finally, 4-PBA significantly improved semen parameters in HFD rats (P < 0.05).
CONCLUSION:HFD exposure induced detrimental effects on spermatogenesis, semen quality, serum T level, and testicular cell apoptosis in rats. Treatment with 4-PBA ameliorated HFD？induced impaired spermatogenesis via inhibition of apop-tosis in rats. 4-PBA may have therapeutic value in the treatment of obesity？related impairment of spermatogenesis.