Bloodstream infections with O16-ST131 and O25b-ST131: molecular epidemiology, phylogenetic analysis and antimicrobial resistance.
10.12122/j.issn.1673-4254.2018.12.20
- Author:
Yiming ZHONG
1
;
Xiaohe ZHANG
2
;
Wenen LIU
1
;
Fang YANG
1
;
Qun YAN
1
;
Qingxia LIU
1
;
Yanming LI
1
;
Hongling LI
1
;
Mingxiang ZOU
1
Author Information
1. Department of Clinical Laboratory, Xiangya Hospital, Central South University, Changsha 410008, China.
2. Department of Laboratory Medicine, Xiangya School of Medicine, Central South University, Changsha 410013, China.
- Publication Type:Journal Article
- Keywords:
Escherichia coli;
ST131;
antimicrobial resistance;
bloodstream infection;
clinical features;
phylogenetic groups
- MeSH:
Anti-Bacterial Agents;
therapeutic use;
Bacteremia;
drug therapy;
epidemiology;
microbiology;
China;
Drug Resistance, Bacterial;
Escherichia coli;
classification;
drug effects;
genetics;
Escherichia coli Infections;
drug therapy;
epidemiology;
microbiology;
Genotype;
Humans;
Microbial Sensitivity Tests;
Molecular Epidemiology;
Phylogeny;
Species Specificity
- From:
Journal of Southern Medical University
2018;38(12):1521-1526
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the phylogenetics and prevalence of bloodstream infections with ST131, the antimicrobial resistance profiles of the pathogens, and the clinical features.
METHODS:Non-duplicate isolates were collected from 144 patients with bloodstream infections in our hospital between January and December, 2016.The phylogenetic groups of the isolates were analyzed using multiplex PCR, and O serotyping of ST131 strains was performed by allele-specific PCR.The clinical characteristics of the 144 patients were analyzed to define the differences in the clinical features between patients with ST131 infection and those with non-ST131 infection.Antibiotic susceptibility of the isolates was determined using the Vitek 2 compact system.
RESULTS:The phylogenetic group analysis showed a domination by group B2 (41.0%[59/144]), followed by group F, group B1 and group E, which accounted for 16.7%(24/144), 13.9%(20/144), and 13.2% (19/144), respectively.Nine strains (6.3%) of were identified to be ST131 strains, among which 8 were O25b-B2-ST131 strains and 1 was O16-B2-ST131 strain.Of the 9 cases of ST131 infection, 7(77.8%) were found to occur in a nosocomial setting.The demographic characteristics and clinical features of the ST131-infected patients were similar to those of non-ST131-infected patients.ST131 strains were sensitive to piperacillin/tazobactam, imipenem, ertapenem, and amikacin, but showed high resistance rates to cefazolin, ceftriaxone, ciprofloxacin, levofloxacin, gentamicin, and trimethoprim/ sulfamethoxazole (all over 50%).The positivity rate of ESBLs in the ST131 strains was 77.8%, and the multidrug resistance rate reached 88.9%, which was higher than that of non-ST131 isolates, but the difference was not statistically significant.
CONCLUSIONS:The most common phylogenetic groups of isolates from patients with bloodstream infections are group B2 and F, and the positivity rate of ST131 is low.We for the first time detected O16-ST131 in patients with blood-borne infections in China.The clinical features of ST131-infected patients are similar to those of non-ST131-infected patients.The positivity rate of ESBLs and the multidrug resistance rate are high in ST131 strains, which may raise concerns in the future.
