Effect of methanol-ethyl acetate partitioned fractions from on proliferation and apoptosis of human non-small cell lung cancer H1975 cells.
10.12122/j.issn.1673-4254.2019.02.07
- Author:
Jiahui GUI
1
;
Meilin ZHU
1
;
Xiangjian BAI
1
;
Bohan LI
1
;
Meijia GAO
1
;
Hui MA
1
;
Hongmei LI
1
;
Chengzhu WU
1
Author Information
1. School of Pharmacy, Bengbu Medical College, Bengbu 233030, China.
- Publication Type:Journal Article
- Keywords:
Descurainia sophia;
apoptosis;
cytotoxicity;
human non-small cell lung cancer
- MeSH:
Acetates;
Animals;
Antineoplastic Agents, Phytogenic;
isolation & purification;
pharmacology;
Apoptosis;
drug effects;
Carcinoma, Non-Small-Cell Lung;
pathology;
Cell Line, Tumor;
Cell Proliferation;
drug effects;
Heterografts;
Humans;
Lung Neoplasms;
pathology;
Methanol;
Mice;
Mice, Nude;
Plant Extracts;
isolation & purification;
pharmacology
- From:
Journal of Southern Medical University
2019;39(2):169-174
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effects of methanol-ethyl acetate partitioned fractions from (MEDS) on the proliferation and apoptosis of human non-small cell lung cancer H1975 cells.
METHODS:The systemic solvent extraction method was used to preliminary separation of the effective fractions in the methanol extract of . The cytotoxicity of each extract (5, 10, 20, 40, and 80 μg/mL) was tested using MTT assay. Colony cloning method was used to assess the effect of different concentrations of methanol-ethyl acetate partitioned fractions from MEDS (5, 10, 20, 40, and 80 μg/ mL) on the proliferation of H1975 cells. Flow cytometric analysis with Annexin V-FITC/PI staining was performed to detect the apoptosis of the cells after treatment with different concentrations of MEDS fractions (10, 20, and 40 μg/mL). Western blotting was used to evaluate the effects of MEDS fractions on the expressions of apoptosis-related proteins Akt, Bax, and Bcl-2. The anti-tumor activity of 100 mg/kg MEDS fractions was tested in a nude mouse model bearing H1975 cell xenografts.
RESULTS:MTT assay and colony forming experiment showed that MEDS fractions significantly inhibited the proliferation of H1975 cells in a dose-and time-dependent manner ( < 0.05). The results of flow cytometry showed that MEDS fractions induced obvious apoptosis of H1975 cells in a concentration-dependent manner ( < 0.05). MEDS fractions also significantly decreased the expressions of Bcl-2 and Akt protein and increased the protein expression of Bax ( < 0.05). In the tumor-bearing nude mouse model, MEDS fractions showed potent anti-tumor effects with a low toxicity to affect the body weight and organs of the mice.
CONCLUSIONS:The methanol-ethyl acetate partitioned fractions from MEDS show potent anti-tumor activity both and , suggesting their value as promising therapeutic agents against lung cancer.
