Mimotopes for Api g 5, a Relevant Cross-reactive Allergen, in the Celery-Mugwort-Birch-Spice Syndrome.
10.4168/aair.2016.8.2.124
- Author:
Anna LUKSCHAL
1
;
Julia WALLMANN
;
Merima BUBLIN
;
Gerlinde HOFSTETTER
;
Nadine MOTHES-LUKSCH
;
Heimo BREITENEDER
;
Isabella PALI-SCHOLL
;
Erika JENSEN-JAROLIM
Author Information
1. Department of Comparative Medicine, Messerli Research Institute of the University of Veterinary Medicine Vienna, the Medical University of Vienna and the University of Vienna, Vienna, Austria. erika.jensenjarolim@meduniwien.ac.at
- Publication Type:Original Article
- Keywords:
Celery-mugwort-birch-spice syndrome;
IgE Epitopes;
food hypersensitivity;
high molecular weight (HMW) allergens;
mimotope;
vaccination
- MeSH:
Allergens;
Animals;
Apiaceae;
Apium graveolens;
Artemisia;
Bacteriophages;
Betula;
Carbohydrates;
Clone Cells;
Enzyme-Linked Immunosorbent Assay;
Food Hypersensitivity;
Glycoproteins;
Horseradish Peroxidase;
Humans;
Immune Sera;
Immunization;
Immunoglobulin E;
Immunoglobulin G;
Mice;
Molecular Weight;
Pollen;
Spices;
Vaccination;
Virtues
- From:Allergy, Asthma & Immunology Research
2016;8(2):124-131
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: In the celery-mugwort-birch-spice syndrome, a significant proportion of IgE is directed against high molecular weight (HMW) glycoproteins, including the celery allergen Api g 5. BIP3, a monoclonal antibody originally raised against birch pollen, recognizes HMW allergens in birch and mugwort pollens, celery, and Apiaceae spices. Our aim was to generate mimotopes using BIP3 for immunization against the HMW allergens relevant in the celery-mugwort-birch-spice cross reactivity syndrome. METHODS: Mimotopes were selected from a random-peptide display library by BIP3 and applied in IgE inhibition assays. The 3 phage clones with the highest inhibitory capacity were chosen for immunization of BALB/c mice. Mouse immune sera were tested for IgG binding to blotted birch pollen extract and used for inhibiting patients' IgE binding. Furthermore, sera were tested for binding to Api g 5, to horseradish peroxidase (HRP) as a second glycoprotein, or to non-glycosylated control allergen Phl p 5 in ELISA, and the specific Api g 5-specific IgG titers were determined. RESULTS: Three rounds of biopanning resulted in phage clones exhibiting 7 different sequences including 1 dominant, 1-6-cyclo-CHKLRCDKAIA. Three phage clones had the capacity to inhibit human IgE binding and induced IgG to the HMW antigen when used for immunizing BALB/c mice. The induced BIP3-mimotope IgG reached titers of 1:500 specifically to Api g 5, but hardly reacted to glycoprotein HRP, revealing a minor role of carbohydrates in their epitope. CONCLUSIONS: The mimotopes characterized in this study mimic the epitope of BIP3 relevant for Api g 5, one of the cross-reactive HMW allergens relevant in the celery-mugwort-birch-spice syndrome. BIP3 mimotopes may be used in the future for hyposensitization in this clinical syndrome by virtue of good and specific immunogenicity.
