Anti-tumor and immune-modulating effect of decoction in mice bearing hepatoma H22 tumor.
10.12122/j.issn.1673-4254.2019.02.18
- VernacularTitle:加味四君子汤对H22肝癌小鼠的抑瘤作用和免疫功能的影响
- Author:
Limei CHEN
1
;
Tong JIN
1
;
Chuntao NING
2
;
Suli WANG
3
;
Lijie WANG
2
;
Jingming LIN
2
Author Information
1. School of Chinese Medicine, Southern Medical University, Guangzhou 510515, China.
2. Department of Pharmacy, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China.
3. Guangzhou Baiyun Mountain Pangaoshou Pharmaceutical Co. Ltd., Guangzhou 511400, China.
- Publication Type:Journal Article
- Keywords:
H22 tumor-bearing mice;
Jiawei Sijunzi decoction;
antitumor activity;
immunity
- MeSH:
Animals;
Antineoplastic Agents, Phytogenic;
pharmacology;
Carcinoma, Hepatocellular;
drug therapy;
immunology;
metabolism;
pathology;
Drugs, Chinese Herbal;
pharmacology;
Kidney;
drug effects;
Liver;
drug effects;
pathology;
Liver Neoplasms;
drug therapy;
immunology;
metabolism;
pathology;
Mice;
Necrosis;
Neoplasm Proteins;
metabolism;
Random Allocation;
Up-Regulation
- From:
Journal of Southern Medical University
2019;39(2):241-248
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the antitumor activity of decoction and study its liver and kidney toxicity and its effect on the immune system in a tumor-bearing mouse model.
METHODS:Hepatoma H22 tumor-bearing mouse models were randomized into model group, cyclophosphamide (CTX) group, and low-, moderate-, and high-dose decoction groups (JW-L, JW-M, and JW-H groups, respectively). The antitumor activity of decoction was assessed by calculating the tumor inhibition rate and pathological observation of the tumor tissues. Immunohistochemistry was used to detect the expressions of Bax, Bcl-2, Bax/Bcl-2 and caspase-3 in the tumors. The liver and kidney toxicity of decoction was analyzed by evaluating the biochemical indicators of liver and kidney functions. The immune function of the tumor-bearing mice were assessed by calculating the immune organ index, testing peripheral blood routines, and detection of serum IL-2 and TNF-α levels using enzyme-linked immunosorbent assay.
RESULTS:Compared with that in the model group, the tumor mass in CTX, JW-M and JW-H groups were all significantly reduced ( < 0.05) with cell rupture and necrosis in the tumors. Immunohistochemistry revealed obviously up-regulated expressions of Bax and caspase-3 and down- regulated expression of Bcl-2 protein with an increased Bax/Bcl-2 ratio in CTX, JW-M and JW-H groups. Treatment with decoction significantly reduced Cr, BUN, AST and ALT levels, improved the immune organ index, increased peripheral blood leukocytes, erythrocytes and hemoglobin levels, and up-regulated the levels of TNF-α and IL-2 in the tumor-bearing mice. These changes were especially significant in JW-H group when compared with the parameters in the model group ( < 0.01).
CONCLUSIONS: decoction has a strong anti-tumor activity and can improve the liver and kidney functions of tumor-bearing mice. Its anti-tumor effect may be attributed to the up-regulation of Bax, caspase-3, TNF-α and IL-2 levels and the down-regulation of Bcl-2 expression as well as the enhancement of the non-specific immune function.
