Evaluation of renal oxygenation in rats with acute aristolochic acid nephropathy using blood oxygenation level-dependent magnetic resonance imaging.
10.12122/j.issn.1673-4254.2019.05.05
- Author:
Guixiang YANG
1
;
Yingjie MEI
2
;
Jian LÜ
2
;
Quan TAO
2
;
Yanqiu FENG
2
;
Yikai XU
1
Author Information
1. Medical Imaging Center, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
2. School of Biomedical Engineering, Guangdong Provincial Key Laboratory of Medical Image Processing, Southern Medical University, Guangzhou 510515, China.
- Publication Type:Journal Article
- Keywords:
R2* values;
aristolochic acid;
blood oxygenation level-dependent magnetic resonance imaging
- MeSH:
Animals;
Aristolochic Acids;
Kidney;
Kidney Diseases;
metabolism;
Magnetic Resonance Imaging;
Oxygen;
Random Allocation;
Rats;
Rats, Wistar
- From:
Journal of Southern Medical University
2019;39(5):528-532
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To evaluate the changes in renal oxygenation in rats with acute aristolochic acid nephropathy using blood oxygenation level-dependent (BOLD) magnetic resonance imaging (MRI) at 7.0T.
METHODS:Wistar rats were randomly divided into AAN group (=18) and control group (=6) for intraperitoneal injections of AAI at 40 mg/kg and PEG400, respectively, on a daily basis for 6 consecutive days. All the control rats and 6 rats from AAN group underwent BOLD MRI scan before and at 2, 4, and 6 days after the initial injection for measuring renal cortical and medullary R2 values. At each of the 4 time points, 3 rats in AAN group were sacrificed for histological evaluation; the control rats were examined at 6 days after the initial injection.
RESULTS:The cortical and medullary R2 values of the rats in AAN group on days 4 and 6 were significantly higher than those in the control group ( < 0.05). In AAN group, the cortical R2 values showed no obvious changes on day 2 as compared with the baseline values, but increased significantly on day 4 ( < 0.05) and day 6 ( < 0.01); the medullary R2 values increased progressively and were significantly higher than the baseline values on day 4 ( < 0.01) and day 6 ( < 0.01). In the control group, no significant changes were detected in either cortical or medullary R2 values throughout the experiment.
CONCLUSIONS:BOLD MRI allows non-invasive measurement of renal oxygenation levels in rats with AAN. The increase of renal cortical and medullary R2 values, and particularly the latter, indicates a lowered renal oxygenation level, which provides potentially useful information for clinical decisions.
