Mig- gene silencing inhibits vasculogenic mimicry formation and invasion of glioma U251 cells by suppressing MEK/ERK signaling.
10.12122/j.issn.1673-4254.2019.05.11
- Author:
Fan WANG
1
;
Fenglong CHEN
1
;
Weipeng HU
2
;
Yi ZHANG
1
Author Information
1. Department of Neurosurgery, Third Hospital of Xiamen, Xiamen 361100, China.
2. Department of Neurosurgery, Second Affiliated Hospital of Fujian Medical University, Quanzhou 362000, China.
- Publication Type:Journal Article
- Keywords:
Mig-7;
RNA interference;
glioma;
tumor invasion;
vasculogenic mimicry
- MeSH:
Cell Line, Tumor;
Cell Movement;
Cell Proliferation;
Gene Silencing;
Glioma;
genetics;
pathology;
Humans;
Neoplasm Proteins;
metabolism;
RNA, Small Interfering;
Signal Transduction
- From:
Journal of Southern Medical University
2019;39(5):566-571
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the inhibitory effects of silencing migration-inducing gene-7 (Mig-7) on vasculogenic mimicry formation, migration and invasion of human glioma cells and whether MEK/ERK signaling pathway mediates these effects.
METHODS:Human glioma U251 cells were infected by lentiviral vectors carrying a small interfering RNA targeting Mig-7 gene (sh-Mig-) or a negative control shRNA (sh-NC), and real-time quantitative PCR was used to detect the expression level of Mig- mRNA in the cells. Three-dimensional culture and Transwell chamber invasion assay were used to observe the effect of Mig- gene silencing on vasculogenic mimicry formation and invasion ability of the U251 cells. Western blotting was performed to detect the changes in the protein expression levels of MEK/ERK in the infected cells.
RESULTS:We successfully obtained a U251 cell line with stable low expression of Mig- gene using RNA interference technique. Compared with the cells infected with sh-NC lentivirus and the non- infected cells, U251 cells infected with the lentiviral vector carrying sh-Mig- showed significantly decreased expression level of Mig- ( < 0.01) with obviously lowered vasculogenic mimicry formation and invasion abilities ( < 0.05). Mig- silencing also significantly lowered the expressions of MEK and ERK proteins in U251 cells ( < 0.05).
CONCLUSIONS:Silencing of Mig-7 gene inhibits vasculogenic mimicry formation and invasion of U251 cells possibly by suppressing MEK/ERK signaling, suggesting the important role of Mig-7 gene in vasculogenic mimicry formation and invasion of human glioma cells.
