Correlation between special A/O genotype and the O phenotype.
10.3760/cma.j.issn.1003-9406.2019.03.021
- Author:
Hong LUO
1
;
Runqing ZHANG
;
Zhijian LIAO
;
Chunyan MO
;
Ling WEI
;
Mingyue LIANG
;
Zhao XIE
;
Guangping LUO
Author Information
1. Institute of Clinical Transfusion Medicine, Guangzhou Blood Center, Guangzhou, Guangdong 510095, China. tigerluo@126.com.
- Publication Type:Journal Article
- MeSH:
ABO Blood-Group System;
Alleles;
Exons;
Genotype;
Humans;
Phenotype
- From:
Chinese Journal of Medical Genetics
2019;36(3):275-277
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the correlation between special A/O genotype and the O phenotype.
METHODS:Group O samples with partially reduced or lack of isoagglutinins were collected to determinate the ABO genotype with a PCR-sequence specific primer (PCR-SSP) assay. Seven samples with A/O genotype were selected for further study. Serological tests including forward and reverse typing, H antigen determination and adsorption/elution were carried out with a tube method. Genomic DNA was genotyped by amplifying and sequencing of the coding regions of exons 1 to 7 of the ABO gene.
RESULTS:Seven samples were serotyped as group O by the forward typing test. However, reduced anti-A activity was found in 5 samples by the reverse typing test, reduced anti-A and anti-B activities were found in 1 sample, and no anti-A isoagglutinin activity was found with 1 sample. H antigen was determined in all samples by routine serologic method. Neither anti-A nor anti-B was eluted from red cells derived from all samples. Three samples were genotyped as Ael02/O02, whilst the remainders were Ael02/O13, Ael02/O65, Am04/O75, Ael06/O02, respectively.
CONCLUSION:Special A/O genotype may not express the A antigen, leading to the generation of group O red cells. Reduced or missed anti-A activity is the typical serological feature of this special group of O phenotype, for which ABO*Ael02 and ABO*O02 are the major alleles. Group O individuals with isoagglutinin detection problem should be grouped by serological tests and genomic DNA analysis.
