Detection of chromosomal abnormalities in abortic tissues derived from patients with recurrent abortions using BoBs technique.
10.3760/cma.j.issn.1003-9406.2019.05.022
- Author:
Xin JIANG
1
;
Shuqin ZHAO
;
Xiujie PAN
;
Xuemei QIU
;
Xin NIU
;
Mei LI
;
Xiaoli XIE
;
Shiya LIU
Author Information
1. Reproductive and Genetic Center, Maternal and Child Health Care Hospital of Zaozhuang, Shandong 277100, China. Email: zzzsq110@163.com.
- Publication Type:Journal Article
- MeSH:
Abortion, Habitual;
Abortion, Spontaneous;
Adult;
Chromosome Disorders;
Down Syndrome;
Female;
Humans;
Karyotyping;
Pregnancy;
Prenatal Diagnosis
- From:
Chinese Journal of Medical Genetics
2019;36(5):502-504
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To assess the value of BACs-on-Beads (BoBs) technique for the detection of chromosomal abnormalities in abortus tissues from recurrent pregnancy loss.
METHODS:A total of 109 abortus samples were collected and analyzed with the BoBs technique. The incidence and types of chromosomal abnormalities for different age groups and gestational weeks were compared.
RESULTS:The BoBs assay has succeeded in all cases, with an incidence for chromosomal abnormalities reaching 62.39% (68/109). The major findings included trisomy 16 (12/68), trisomy 22 (9/68), trisomy 13 (9/68) and trisomy 21 (8/68). The abnormal rate was significantly higher in those above 35-year-old compared with that of the <35-year-old group (P<0.05). More aberrations were found among abortus tissues derived from 42-70 days of pregnancy albeit with no statistical significance (P>0.05). The aberration rates were similar for samples derived from second, third and fourth time abortions (P>0.05).
CONCLUSION:BoBs technique can detect chromosomal aberrations in miscarriages and may be routinely used for the analysis of early spontaneous abortions.
