Effect of siRNA-Interfering β-Catenin Expression on MDR of Human Multiple Myeloma Cell Line.
10.19746/j.cnki.issn.1009-2137.2019.02.027
- Author:
Jian-Guo ZHANG
1
;
Yu-Peng LI
1
;
Zi-Qing FANG
1
;
Xing-Ru HUANG
2
;
Xu CHEN
2
;
Jian FANG
3
Author Information
1. Third Clinical Medical College of Guangdong University of Traditional Chinese Medicine, Guangzhou 510000, Guangdong Province, China.
2. Department of Orthopedics, The Third Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510000, Guangdong Province, China.
3. Department of Orthopedics, The Third Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510000, Guangdong Province, China,E-mail: 1758305466@qq.com.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
Cell Line, Tumor;
Cell Proliferation;
Humans;
Multiple Myeloma;
RNA, Small Interfering;
beta Catenin;
genetics
- From:
Journal of Experimental Hematology
2019;27(2):477-481
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effecr of siRNA-interfering β-catenin expression on drug-resistance of multiple myeloma cells.
METHODS:The multiple myeloma cell line RPMI-8226 was cultured in vitro. The maphalan-resistant cell model was established by concentration gradient ascending of durg, then the drug-resistant cell line was instantaneously transfected with β-catenin siRNA, the sensitivity of RPMI 8226 cells to maphalan was detected by CCK-8 meltod before and after the transfection with siRNA; the mRNA and protein expression of β-catenin was detected by qRT-PCR and Western blot respectively, the apoptosis of cells was detected by flow cytometry.
RESULTS:IC of maphalan decreased from (5.29±0.19) μmol/L to (1.88±0.64) μmol/L, suggesting that the deplation of β-eatenin restored the sensitivity of drug-resistant cell line RPMI-8226 to malphalan. The Western blot showed that after the instaintaneous transfection with β-catenin siRNA, the β-catenin protein expression level obviously decreased, compared with level before transfection. After transfection, the maplalan-inducing apoptosis rate of cells increased from (35±0.5)% to (54±0.4)%, suggesting that the β-catinin gene may correlated with drug-resistance of cells. Interfering the expression of β-catenin gene could enhance the sensitivity of drug-resistant RPMI-8226 cells to maphalan.
CONCLUSION:The β-catenin siRNA interfereuce can inhisit the β-catenin gene expression in Wnt/β-catenin signaling pathway, suppress the cell proliferation, enhence the toxicity of maphalan on drug-resistant RPMI-8226 cells, thus result in increase of cell apoptosis.
