Instability of Mitochondrial DNA D-loop Region Genes in Patients with Leukemia.
10.19746/j.cnki.issn.1009-2137.2019.03.005
- Author:
Jing WANG
1
;
Yong-An ZHOU
2
;
Li-Ping SU
3
;
Feng-Min LI
1
;
Ming CHEN
1
Author Information
1. Department of Hematology, The First Hosptial of Qinhuangdao City, Qinhuangdao 066000, Hebei Province, China.
2. Department of Laboratorial Examination, Taiyuan Center Hospital, Taiyuan 030009, Shanxi Province, China,E-mail: 254090720@qq.com.
3. Department of Hematology, Shanxi Provincial Cancer Hospital, Taiyuan 030013, Shanxi Province, China.
- Publication Type:Journal Article
- MeSH:
DNA, Mitochondrial;
Humans;
Leukemia;
Mitochondria;
Mutation;
Mutation Rate
- From:
Journal of Experimental Hematology
2019;27(3):657-663
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the instability of mitochondrial DNA（mt DNA） D-loop region genes in patients with Leukemia.
METHODS:The HV-1 and HV-2 regions of D-loop region in 24 patients with leukemia were amplificated and sequenced, then their results were compared with revised Cambridge reference sequence (rCRS) and Databank mtDB. The mutation rate was detected by SPSS 22.0 statistics software.
RESULTS:The total mutation rate in patients was 95.83% (23/24), the detection showed 82 mutated genes, out of which 47 (57.32%) mutated genes located in HV-1 region, 35 (42.68%) mutated genes in HV-2 region. The comparison showed that the mutation rate in untreated (UT) group and treated (T) group of AML patients was (2.37±0.82)×10 and (4.76±2.45)×10 respectively(P＜0.01), the mutation rate in PR and CR groups of treated AML patients was (5.10±2.56)×10 and (4.51±2.51)×10 respectively (P＜0.05), the comparison among M3 group showed that the mutation rates in UT, PR and CR groups were (2.55±0.63)×10, (5.37±3.41)×10 and (3.71±1.65)×10 respectively (P＞0.05).
CONCLUSION:The more high mutation rate and many kinds of mutation types exist in D-loop region, suggesting that the genes in D-loop region display the more strong instability, the chemotherapy may aggravate the instability of genes in D-loop region.
