Expression Difference of RhoH Gene in Leukemia and Its Clinical Significance.
10.19746/j.cnki.issn.1009-2137.2019.03.019
- Author:
Jun NI
1
;
Qing-Qing SHI
1
;
Wei WU
1
;
Xin SUN
1
;
Yue-Zhi FANG
1
;
Jian GU
2
Author Information
1. Laboratory of Blood Diseases and Hematonosis, Jiangsu Northern People's Hospital, Yangzhou Institute of Hematology, Yangzhou 225001, Jiangsu Province, China.
2. Laboratory of Blood Diseases and Hematonosis, Jiangsu Northern People's Hospital, Yangzhou Institute of Hematology, Yangzhou 225001, Jiangsu Province, China,E-mail: maolujiu918@163.com.
- Publication Type:Journal Article
- MeSH:
Acute Disease;
Bone Marrow;
Humans;
Leukemia, Myeloid, Acute;
genetics;
Prognosis;
RNA, Messenger;
Transcription Factors;
genetics;
rho GTP-Binding Proteins;
genetics
- From:
Journal of Experimental Hematology
2019;27(3):758-762
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the expression and clinical significance of RhoH gene in bone marrow cells of leukemia patients.
METHODS:31 cases of leukemia and 15 cases of non-tumor as controls were collected. The expression of RhoH in bone marrow cells was detected by real-time quantitative PCR (RQ-PCR). The median expression level of RhoH was used as the cut-off value. The newly diagnosed patients were divided into RhoH high expression group and low expression group. The relationship of different RhoH expression levels with clinical features and prognosis of newly diagnosed patients was analyzed.
RESULTS:The mRNA expression of RhoH in the bone marrow cells of 31 cases of leukemia was significantly lower than that in the control group, mRNA expression of RhoH in the ALL group was significantly lower than that in AML group (P＜0.05). Compared with the RhoH high expression group, the proportion of bone marraw blasts and LDH level in the RhoH low expression group was significantly increased (P＜0.05), but there were no significant differences in clinical features such as age, white blood cell count, hemoglobin level, platelets count, PCT and CRP level (P＞0.05). In AML, the recurrence rate after standard chemotherapy in RhoH low expression group was higher than that in high expression group, while the expression of RhoH not correlated with other prognostic genes of AML. In ALL, the recurrence rate in RhoH low expression group was not statistically significant different from that in high expression group.
CONCLUSION:RhoH may be involved in the genesis of acute leukemia. In AML, RhoH expression negatively correlates with recurrence rate, which can be used as a prognostic indicator independently. In ALL, RhoH may participate in the disease process through other mechanism.
