Efflect of miR-34a-Tangeted Regulation of HDACI on Apoptosis of AML Cells.
10.19746/j.cnki.issn.1009-2137.2019.03.024
- Author:
Chao WEN
1
;
Yuan-Kang XIE
2
;
Yi-Jian CHEN
3
Author Information
1. School of Nursing, Gannan Medical College,The First Affiliated Hospital of Gannan Medical College, Ganzhou 341000, Jiangxi Province, China.
2. Department of Hepatobiliary Surgery, The First Affiliated Hospital of Gannan Medical College, Ganzhou 341000, Jiangxi Province, China.
3. Department of Hematology, The First Affiliated Hospital of Gannan Medical College, Ganzhou 341000, Jiangxi Province, China,E-mail: chenyj2005@163.com.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
Cell Proliferation;
HL-60 Cells;
Histone Deacetylase 1;
metabolism;
Humans;
Leukemia, Myeloid, Acute;
genetics;
MicroRNAs;
genetics
- From:
Journal of Experimental Hematology
2019;27(3):790-795
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the regulation of miR-34a on HDAC1 expression and its effect on the apoptosis of acute myeloid leukemia (AML) cells.
METHODS:miR-34a mimics, miR-34a inhibitor and miR-34a scramble were transfected into HL-60 cells. The effects of miR-34a expression levels on proliferation and apoptosis of HL-60 cell were detected by CCK8 assay and flow cytometry respectively. The expression of HDAC1 protein was assessed by Western blot after regulating miR-34a expression, the 3'UTR of HDAC1 was cloned and ligated to construct a dual luciferase reporter vector, and then the dual luciferase reporter assay was applied to verify the target of miR-34a, the expression vector pcDNA3.1-HDAC1 was constructed, the interaction of miR-34a and HDAC1 was analyzed by reversion test.
RESULTS:miR-34a over-expression could inhibit the proliferation of HL-60 cells and induce their apoptosis. Bioinformatics analysis indicated that the HDAC1 was a target gene of miR-34a. Western blot indicated that miR-34a overexpression down-regulated the expression of HDAC1. Dual luciferase reporter assay and reversion test showed that miR-34a could act at the 3-UTR of HDAC1 gene to regulate its expression.
CONCLUSION:miR-34a promotes the apoptosis of HL-60 cells via regulating HDAC1 expression.
