Expression of MiR101 and EZH2 in Patients with Mantle Cell Lymphoma and Its Clinical Significance.
10.19746/j.cnki.issn.1009-2137.2019.03.029
- Author:
Yan-Ling LIN
1
;
Zong-Kai ZOU
1
;
Hai-Yan SU
1
;
Yi-Qun HUANG
2
Author Information
1. Department of Pathology,Zhangzhou Hospital Affiliated to Fujian Medical University, Zhangzhou 363000, Fujian Province, China.
2. Department of Hematology, Zhangzhou Hospital Affiliated to Fujian Medical University, Zhangzhou 363000, Fujian Province, China,E-mail: nchuangyiqun@126.com.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
Cell Proliferation;
Enhancer of Zeste Homolog 2 Protein;
genetics;
Humans;
Lymphoma, Mantle-Cell;
genetics;
MicroRNAs;
genetics;
Prognosis
- From:
Journal of Experimental Hematology
2019;27(3):820-826
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the expression of miR-101 and EZH2 in patients with mantle cell lymphoma(MCL) and to analyze its correlation with clinical prognosis of MCL patients.
METHODS:RQ-PCR and S-P immunohistochemistry were used to detect the expressions of miR-101 and EZH2 in tissue of MCL patients. CCK-8 was used to assay the effect of miR-100 minics on the proliferation of Jeko-1 and Mino cells; the flow cytometry with Annexin V/PI double staining was used to assay the apoptosis; Western blot was used to assay the effect of miR-101 minics on the expression of EZH2 protein in Jeko-1 and Mino cells.
RESULTS:Compared with control group, miR-101 lowly expressed, and EZH2 protein highly expressed in MCL group, with very statistically significant difference(P<0.01).There was negative correlation between miR-101 and EZH2 expression(r=-0.638，P<0.05). The expression of miR-101 and EZH2 significantly correlated with B symptoms, International Prognostic Index(IPI) and Ann Arbor stage, respectively. Survival analysis showed that the overall survival(OS) rate of patients with low expression of miR-101 were significantly lower than that of patients with high miR-101 expression (P=0.0014), the OS rate of patients with EZH2 high expression were significantly lower than that of patients with EZH2 low expression (P=0.0093). The miR-100 minics could inhibit the proliferation of Jeko-1 and Mino cells, and increase the apoptotic rate. The expression of EZH2 protein was markedly suppressed by the miR-100 minics.
CONCLUSION:The expression of miR-101 and EZH2 is different in MCL patients with different clinical stage and prognosis. The miR-101 can inhibit the cell proliferation and induce cell apoptosis of mantle cell lymphoma by targeting EZH2.
