Oxidative Damage of Bone Marrow Stromal Cells Caused by Chemotherapy Drugs.
10.7534/j.issn.1009-2137.2019.03.054
- Author:
Zhe LIU
1
;
Yi-Hui LI
1
;
Zhen-Ya XUE
1
;
Ke-Jing TANG
1
;
Ying-Xi XU
1
;
Hai-Yan XING
1
;
Zheng TIAN
1
;
Min WANG
1
;
Qing RAO
2
Author Information
1. State Key Laboratory of Experimental Hematology，Institute of Hematology ＆Blood Diseases Hospital，Chinese Academy of Medical Sciences ＆ Peking Union Medical College，Tianjin 300020，China.
2. State Key Laboratory of Experimental Hematology，Institute of Hematology ＆Blood Diseases Hospital，Chinese Academy of Medical Sciences ＆ Peking Union Medical College，Tianjin 300020，China,E-mail:raoqing@ihcams.ac.cn.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
Daunorubicin;
Mesenchymal Stem Cells;
Oxidative Stress;
Reactive Oxygen Species
- From:
Journal of Experimental Hematology
2019;27(3):970-975
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the oxidative damage of OP9 cells induced by daunorubicin (DNR) treatment.
METHODS:The TMRM probe was used to detect mitochondrial membrane potential by flow cytometry; the reactive oxygen species (ROS) was determined by flow cytometry DCFDA probe; the real-time PCR was used to detect the molecular expression of antioxidant enzyme，glutathione peroxidase (GPX) in OP9 cells; the expression of γ-H2AX was determined by flow cytometry.
RESULTS:Compared with normal OP9 cells, the positive rate of TMRM in DNR-treated OP9 cells decreased by 56.7% (P＜0.05); the positive rate of DCFDA in DNR-treated OP9 cells increased by 3.52 times (P＜0.01). Compared with normal OP9 cells, DNR-treated OP9 cells showed a decrease in the expression of GPX4 by 44.22% (P＜0.001); the expression of GPX7 decreased by 65.7% (P＜0.001); the expression of GPX8 decreased by 24.7% (P＜0.001); the positive rate of γ-H2AX in DNR-treated OP9 cells increased (P＜0.05).
CONCLUSION:After DNR treatment, mitochondrial membrane potential of OP9 cells decreases; the level of reactive oxygen species increases; the expression of glutathione peroxidase (GPX) molecules decreases significantly; genomic instability increases obviously; the oxidative damage of cells increased.
