EPO and EPOR Expression in Patients with Acute Leukemia and Its Clinical Significance.
10.19746/j.cnki.issn.1009-2137.2019.04.006
- Author:
Run-Mei TIAN
1
;
Cheng-Shuang HUANG
1
;
Qiong SU
1
;
Liang SHI
1
;
Xi LUO
1
;
Yan CHEN
2
Author Information
1. Department of Pediatrics, Affiliated Hospital of Zunyi Medical University, Zunyi 563003，Guizhou Province, China.
2. Department of Pediatrics, Affiliated Hospital of Zunyi Medical University, Zunyi 563003，Guizhou Province, China,E-mail: cyz600@ 163.com.
- Publication Type:Journal Article
- MeSH:
Bone Marrow;
Erythropoietin;
Gene Expression;
Humans;
Leukemia, Myeloid, Acute;
Receptors, Erythropoietin
- From:
Journal of Experimental Hematology
2019;27(4):1026-1032
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the expression of erythropoietin (EPO) and erythropoietin receptor (EPOR) in patients with acute leukemia (AL) and its clinical significance.
METHODS:The levels of EPO and EPOR in plasma were determined by ELISA kit. mRNA expression levels of EPO and EPOR were determined by RT-RCR. The protein expression levels of EPO and EPOR were detected by Western blot.
RESULTS:The EPO protein levels in marrow plasma of ALL and AML group were significantly higher than those in the control group (P＜0.05), EPOR protein levels in ALL and AML group were significantly lower than those in the control group (P＜0.05). The mRNA levels of EPO and EPOR in ALL and AML groups were significantly higher than those in the control group (P＜0.05). The mRNA levels of EPO and EPOR in the high risk ALL and AML groups were significantly higher than those in the medium, low risk group and the control group (P＜0.05). The protein expression levels of EPO and EPOR in ALL and AML groups were significantly higher than that in control group (P＜0.05). The mRNA levels of EPO and EPOR in ALL and AML groups did not correlate with hemoglobin level and erythrocyte count (P＞0.05).
CONCLUSION:The expressions of EPO and EPOR is higher in ALL and AML patients. The expression levels of EPO and EPOR relate with the risk of ALL and AML. High risk patients have higher expression levels of EPO and EPOR, however, the expression levels of EPO and EPOR do not correlate with hemoglobin level and erythrocyte counting.
