Expression Levels of JARID1B, Hes1 and MMP-9 Genes in CML Patients Treated with Imatinib Mesylate.
10.19746/j.cnki.issn.1009-2137.2019.04.013
- Author:
Zhi-Kai HE
1
;
Seng XUE
1
;
Yong-Hong ZHANG
1
;
Lin LI
1
;
Yun-Jin XIA
2
;
Xiang WANG
2
;
Xin SHI
3
;
Yu LIU
2
;
Zheng XU
4
;
Chen LI
1
;
Jing-Xuan ZHANG
5
Author Information
1. Institute of Basic Medical Sciences, School of Basic Medical Sciences, Hubei University of Medicine, Shiyan 442000, Hubei Province, China.
2. Department of Hematology, Taihe Hospital Affiliated to Hubei University of Medicine, Shiyan 442000, Hubei Province, China.
3. Department of Hematology, Taihe Hospital Affiliated to Hubei University of Medicine, Shiyan 442000, Hubei Province, China,Medical Research Institute, Wuhan University,Wuhan 430000, Hubei Province, China.
4. Key Laboratory of Targeted Intervention of Cardiovascular Diseases, Department of Pathophysiology, Nanjing Medical University, Nanjing 210000, Jiangsu Province, China.
5. Institute of Basic Medical Sciences, School of Basic Medical Sciences, Hubei University of Medicine, Shiyan 442000, Hubei Province, China,E-mail: zhangjx@hbmu.edu.cn.
- Publication Type:Journal Article
- MeSH:
Antineoplastic Agents;
therapeutic use;
Humans;
Imatinib Mesylate;
therapeutic use;
Jumonji Domain-Containing Histone Demethylases;
Leukemia, Myelogenous, Chronic, BCR-ABL Positive;
drug therapy;
Matrix Metalloproteinase 9;
Nuclear Proteins;
Repressor Proteins;
Transcription Factor HES-1
- From:
Journal of Experimental Hematology
2019;27(4):1071-1076
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the relationship between the expression levels of JARID1B,Hes1 and MMP-9 genes and the stages of chronic myelogenous leukemia(CML) and the curative effect of imatinib mesylate (IM).
METHODS:Peripheral blood samples of 15 cases of CML in chronic phase and 10 cases of CML in progressive phase were collected from the Hematology Department of Taihe Hospital affiliated to Hubei University of Medicine and 15 cases of healthy people in the Physical Examination Center. CML patients were divided into effective group and ineffective group based on the efficacy after treatment with IM, then real-time PCR was used to detect the expression levels of JARID1B, Hes1 and MMP-9 mRNA, finally, the differences in the level of gene expression and their correlations with CML stages and IM curative efficacy were analysed.
RESULTS:The expression levels of Hes1 and MMP-9 in initially diagnosed patients in chronic and progressive phase without IM treatment were significantly higher than those of health people（P＜0.05）. There was no significant difference in the expression level of JARID1B between chronic phase patients and health people（P＞0.05）, but the expression level of JARID1B in the progressive phase patients was higher than that of health people （P＜0.05）. The expression levels of JARID1B and Hes1 in the IM-effective group were not significantly different from those in the IM-ineffective group (P=0.85,P=0.82）, while the expression level of MMP-9 in the IM-effective group [JP2]was significantly lower than that in the IM-ineffective group（P＜0.05）.
CONCLUSION:The expression levels of JARID1B Hes1 and MMP-9 relate with the different phase of CML; The expression levels of JARID1B and Hes1 have not significant relationship with IM curative efficacy, the MMP-9 gene expression level relates with IM curative efficacy.
