Imprinting genes modified parthenogenetic embryonic stem cells produce full-term mouse via tetraploid complementation.

Xu LI; Keli Peng; Jinxin Zhang; Qian Gao; Wenhao Zhang; Ruotong Hua; Ling Shuai

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Imprinting genes modified parthenogenetic embryonic stem cells produce full-term mouse via tetraploid complementation.

Author: Xu LI ¹ ; Keli PENG ¹ ; Jinxin ZHANG ¹ ; Qian GAO ¹ ; Wenhao ZHANG ¹ ; Ruotong HUA ¹ ; Ling SHUAI ¹
Author Information

1. State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, Nankai University, Tianjin 300350, China.
Publication Type:Journal Article
Keywords: gene editing; imprinting genes; parthenogenetic embryonic stem cells; tetraploid compensation
MeSH: Animals; Embryonic Stem Cells; Gene Knockout Techniques; Genomic Imprinting; Mice; Parthenogenesis; Pluripotent Stem Cells; Regenerative Medicine; Tetraploidy
From: Chinese Journal of Biotechnology 2019;35(5):910-918
CountryChina
Language:Chinese
Abstract: Parthenogenetic embryonic stem cells (pESCs) derived from bi-maternal genomes do not have competency of tetraploid complementation, due to lacking of paternal imprinting genes. To make pESCs possess fully development potentials and similar pluripotency to zygote-derived ESCs, we knocked out one allelic gene of the two essential maternal imprinting genes (H19 and IG) in their differentially methylated regions (DMR) via CRISPR/Cas9 system and obtained double knock out (DKO) pESCs. Maternal pESCs had similar morphology, expression levels of pluripotent makers and in vitro neural differentiation potentials to zygotes-derived ESCs. Besides that, DKO pESCs could contribute to full-term fetuses through tetraploid complementation, proving that they held fully development potentials. Derivation of DKO pESCs provided a type of major histocompatibility complex (MHC) matched pluripotent stem cells, which would benefit research in regenerative medicine.