Effects of gender and age on protein secretion in saliva of transgenic mice expressing human nerve growth factor.

Sha Liao; Qingchun Zhu; Zhenfang WU; Zicong LI; Fang Zeng

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Effects of gender and age on protein secretion in saliva of transgenic mice expressing human nerve growth factor.

Author: Sha LIAO ¹ ; Qingchun ZHU ¹ ; Zhenfang WU ¹ ; Zicong LI ¹ ; Fang ZENG ¹
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1. College of Animal Science, South China Agricultural University, Guangzhou 510642, Guangdong, China.
Publication Type:Journal Article
Keywords: age; gender; human nerve growth factor; saliva; transgenic mice
MeSH: Animals; Cell Differentiation; Female; Humans; Male; Mice; Mice, Transgenic; Nerve Growth Factor; Saliva
From: Chinese Journal of Biotechnology 2019;35(6):1041-1049
CountryChina
Language:Chinese
Abstract: Nerve growth factor (NGF) can promote the development, differentiation and regeneration of neurons. Recently, in order to efficiently produce human NGF (hNGF) drugs with better efficacy, we created transgenic mice expressing hNGF specifically in their salivary glands, and purified highly active hNGF protein from their saliva. Some studies reported that the NGF secretion in mouse saliva is affected by gender and age. Here, in order to select hNGF transgenic mice with high NGF secretion for saliva collection and hNGF purification, we divided transgenic mice into 4 groups, including 28-day-old young males and females, 63-day-old adult males and females. We compared their saliva volume, total salivary protein amount, salivary mNGF protein amount and salivary hNGF protein amount. The results showed that the saliva volume as well as amounts of total salivary protein, salivary mNGF protein and salivary hNGF protein secreted by 63-day-old transgenic mice were significantly higher than those secreted by sex-match 28-day-old transgenic mice, and the salivary hNGF protein amount secreted by male transgenic mice at the age of 63 days was significantly higher than that of female transgenic mice at the same age; Among 4 groups of mice, 63-day-old male transgenic mice secreted the highest salivary hNGF content, which was about 46 times higher than that secreted by the 28-day-old female transgenic mice. Therefore, 63-day-old male transgenic mice should be selected for saliva collection and hNGF purification.