Chirality Self-assembling peptide for rats endometrial regeneration model.
- Author:
Shuyi WU
1
;
Shijian LAN
1
;
Jing WEN
1
;
Tianxin ZHAO
1
;
Lan HUANG
1
;
Zhongli LUO
1
Author Information
- Publication Type:Journal Article
- Keywords: Chiral Self-assembling peptide; rats model; uterine repair
- MeSH: Animals; Endometrium; Female; HeLa Cells; Humans; Nanofibers; Peptides; Rats; Regeneration
- From: Chinese Journal of Biotechnology 2019;35(6):1079-1087
- CountryChina
- Language:Chinese
- Abstract: Here we investigate the physical and chemical properties of chiral self-assembling peptides and the role of uterine trauma regeneration. The circular dichroism was used to analyze secondary structure of chiral self-assembled peptide, and Congo red staining was used to observe the macroscopic process of peptide self-assembling. Erythrocyte lysis assay was used to examine the cleavage of peptide on cell membrane. The nanofiber scaffolds self-assembled by Chiral self-assembling peptides were used as the three-dimensional culture material to observe the growth effect of Hela cell. CCK-8 (cell counting kit-8) was used to study cell viability level between 2D (2-dimensional) and 3D (3-dimensional) culture environment. Rats endometrium curettage model was founded to evaluate the changes by immunohistochemistry staining and and HE staining. The secondary structure of chiral self-assembling peptides was stable β-sheet, and peptide could form dense membrane structure after 24 hours self-assembling cultured in salt ions. There was no harmful for the cell membrane of the peptide before and after self-assembling. Animal experiments show that chiral self-assembling peptide can significantly reduce the inflammatory response, promote the production of neovascularization, and accelerate the repair process. Chiral self-assembling peptide, as a new type of scaffold material, can construct a three-dimensional cell culture environment and used to repair uterine trauma.
