Progress in rolling circle amplification in biological detection.

Author: Zhongxu ZHAN ¹ ; Ju LIU ¹ ; Bolu CHEN ¹ ; Yizhou TANG ¹ ; Guanhua CHEN ¹ ; Hengyi XU ¹
Author Information

1. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, Jiangxi, China.
Publication Type:Journal Article
Keywords: biological detection; isothermal; rolling circle amplification
MeSH: DNA, Single-Stranded; Nucleic Acid Amplification Techniques
From: Chinese Journal of Biotechnology 2019;35(7):1206-1213
CountryChina
Language:Chinese
Abstract: Rolling circle amplification is a rapid, sensitive and isothermal single-stranded DNA amplification technique that can be used with staining or probes to amplify the detection signal. This technology has been widely used in biological detection and other aspects. The present paper introduces how to design rolling circle amplification, summarize its application in the detection of pathogens, nucleic acid tumor markers, proteins, biological small biomolecules, and viruses in recent years and prospects for future development.