Research progresses in microbial 1,3-1,4-β-glucanase: protein engineering and industrial applications.

Author: Chengtuo NIU ¹ ; Xinyue LI ² ; Xin XU ¹ ; Min BAO ¹ ; Yongxian LI ¹ ; Chunfeng LIU ¹ ; Feiyun ZHENG ¹ ; Jinjing WANG ¹ ; Qi LI ¹
Author Information

1. Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, Jiangsu, China.
2. School of Biotechnology, Jiangnan University, Wuxi 214122, Jiangsu, China.
Publication Type:Journal Article
Keywords: 1,3-1,4-β-glucanase; catalytic property; industrial application; protein engineering; stability
MeSH: Amino Acid Sequence; Glycoside Hydrolases; Models, Molecular; Protein Engineering; Substrate Specificity
From: Chinese Journal of Biotechnology 2019;35(7):1234-1246
CountryChina
Language:Chinese
Abstract: 1,3-1,4-β-glucanase (E.C.3.2.1.73) is an important industrial enzyme which cleave β-glucans into oligosaccharides through strictly cutting the β-1,4 glycosidic bonds in 3-O-substituted glucopyranose units. Microbial 1,3-1,4-β-glucanase belongs to retaining glycosyl hydrolases of family 16 with a jellyroll β-sandwich fold structure. The present paper reviews the industrial application and protein engineering of microbial β-glucanases in the last decades and forecasts the research prospects of microbial β-glucanases.