Biosynthesis of α-lipoic acid in Gluconobacter oxydans increases the production of vitamin C by one-step fermentation.

Author: Yu LIU ¹ ; Enxu WANG ¹ ; Caihui PAN ¹ ; Xiutao DONG ¹ ; Mingzhu DING ¹
Author Information

1. Key Laboratory of Systems Bioengineering (Ministry of Education), Frontier Science Center for Synthetic Biology, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.
Publication Type:Journal Article
Keywords: Gluconobacter oxydans; Ketogulonicigenium vulgare; one-step fermentation; α-lipoic acid
MeSH: Ascorbic Acid; Fermentation; Gluconobacter oxydans; Rhodobacteraceae; Thioctic Acid; biosynthesis
From: Chinese Journal of Biotechnology 2019;35(7):1266-1276
CountryChina
Language:Chinese
Abstract: In a one-step fermentation system of vitamin C production with Gluconobacter oxydans and Ketogulonicigenium vulgare, a functional module of α-lipoic acid biosynthesis was constructed in G. oxydans. The engineered G. oxydans was co-cultured with K. vulgare to enhance the growth and 2-keto-L-gulonic acid (2-KGA) production of K. vulgare. This one-step fermentation system alleviated the growth inhibition during the mono-culture of K. vulgare and strengthened the interaction between the two bacteria. Moreover, the yield of vitamin C precursor (2-KGA) increased to 73.34 g/L (the control group was 59.09 g/L), and the conversion of D-sorbitol to 2-KGA increased to 86.0%. This study provides a new idea for further optimizing the one-step fermentation system of vitamin C production.